2020年6月11日 · Time-lapse videos of RUES2-GLR cells during aggregation (Supplementary Video 2) and elongation (Supplementary Video 3) allowed us to follow the process of symmetry-breaking and segregation of...

2018年5月23日 · As controls, RUES2-GLR grafts derived from hESCs treated with WNT3A, WNT3A plus SB, BMP4, or medium alone exhibited lower survival rates and did not induce chick neural markers (Table 1 and ...

2016年12月15日 · The three embryonic stem cell lines described on the following pages, RUES1, RUES2 and RUES3, are available to the research community upon completion of the order process at www.wicell.org/rues. Click here for a list of transgenic lines available from the Brivanlou Laboratory

RUES2 cells are pluripotent and can differentiate to representatives of the three primary germ layers. See RUES2 Derivation Information. References: Recipients are requested to provide notice of publications citing use of this line to the Provider.

2019年7月1日 · RUES2-GLR expresses mCitrine–SOX2 (mCit–SOX2; active in pluripotency and anterior epiblast), mCerulean–BRA (mCer–BRA; expressed at gastrulation in the primitive streak and mesoderm) and ...

Download scientific diagram | Functional validation of the RUES2-GLR cell line a, Specificity of germ layer reporters. When induced to differentiate to individual germ fates, only the specific ...

进一步地，研究人员使用报告系统（RUES2-GLR13）来鉴定表达中胚层（BRA）、内胚层（SOX17）和神经外胚层（SOX2）标记基因的细胞，并追踪到类原肠胚产生三胚层衍生物的过程 （图2）。 此外，研究人员发现， Nodal 和 WNT信号通路 之间的平衡在决定细胞命运和促进人源类原肠胚延伸方面起着一定的作用。 当给予类原肠胚维甲酸（可以破坏前后轴模式而引起先天畸形）时，类原肠胚的轴向模式和组织基因表达出现异常。 由此也表明，在类原肠胚发育之前和 …

2005年8月30日 · An efficient and reversible transposable system for gene delivery and lineage-specific differentiation in human embryonic stem cells. Cell stem cell. 2009 Sep 4;5 (3):332-42. (reference publication) Merkle FT et al. Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations. Nature. 2017 May 11;545 (7653):229-233.

2020年12月1日 · In our experiments, control RUES2 cells exposed to the striatal differentiation protocol exhibited impairment in the acquisition of an MSN striatal fate, compared to H9 and KOLF2 cells. In particular, the RUES2 line failed to express the MSN regulator DARPP32 while preferentially upregulating MGE-enriched transcripts.

... determine whether ACL could also induce endodermal fate in other hPSCs, we treated both RUES2-GLR Rset hPSCs, which harbour a pluripotent state that is intermediate between pre-implantation...