Spatial and functional separation of mTORC1 signalling in ... - Nature
2024年10月9日 · Bafilomycin A1 (BafA1) is a macrolide antibiotic that blocks lysosomal function by targeting the v-ATPase (vacuolar H + ATPase), causing alkalinization of the lysosomal …
mTORC1 activity licenses its own release from the lysosomal surface
2024年11月21日 · S6K is a downstream target and key effector of mTORC1-dependent processes like protein synthesis. 34 Unlike rapamycin, treatment with two independent S6K …
Autophagic lipid metabolism sustains mTORC1 activity in TSC ... - Nature
2019年11月11日 · Here we show that autophagy supports mTORC1 hyperactivation in neural stem cells (NSCs) lacking tuberous sclerosis complex subunit 1 (Tsc1), thereby promoting …
MORG1 limits mTORC1 signaling by inhibiting Rag GTPases - Cell …
2023年12月15日 · BafA1 is an inhibitor of vacuolar H + ATPase (V-ATPase), and by preventing acidification and subsequent lysosomal degradation of cargoes, it is used to measure …
S6K1 Is Indispensible for Stress-Induced Microtubule Acetylation …
2021年4月17日 · We have previously shown that S6K1 kinase is essential for autophagic flux under stress conditions. Here, we aimed to elucidate the underlying mechanism of S6K1 …
Lysosomes mediate the mitochondrial UPR via mTORC1 …
2023年9月7日 · Strikingly, the half maximal inhibitory concentration (IC 50) of ConA on inhibiting Dox-induced UPR mt is below 1.5 nM in MEFs, while that of BafA1 is around 50 nM (Fig. 1c), …
Nature | mTORC1通路的特异底物激活机制 - 知乎 - 知乎专栏
mTORC1在细胞溶酶体表面激活，磷酸化下游底物： S6K激酶 、 4E-BP1 和转录因子 TFEB 等来促进细胞的合成代谢。 生长因子、氨基酸和葡萄糖能量水平改变都可调控mTORC的活性。生 …
Fatty acid oxidation and autophagy promote endoxifen resistance …
2021年3月23日 · The autophagy inhibitor BafA1, when combined with the AKT inhibitor MK2206, caused a robust sensitization of the cells to endoxifen. This indicates that autophagy activated …
Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity …
2020年2月6日 · Bafilomycin A1 (BafA1), a macrolide, potently inhibits the v-ATPase pump and prevents acidification of lysosomes (Figure 1 A) (Bowman et al., 1988). Both compounds …
mTOR 信号网络中核糖体蛋白 S6 激酶 (S6K) 的调节和功能 …
2012年1月1日 · 核糖体蛋白 S6K（S6 激酶）代表了 TORC1 [TOR（雷帕霉素靶点）复合物 1] 的一种广泛研究的效应物，其在细胞和生物体生理学中具有重要但尚未完全确定的作用。 …