Streptavidin-HRP conjugate is typically used in the immunodetection of biotinylated proteins. This conjugate is suitable for use in immunoblotting, ELISA, immunomicroscopy.

Streptavidin, a bacterial protein isolated from Streptomyces avidinii, is similar to egg-white avidin in its ability to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties.

The streptavidin, horseradish peroxidase (HRP) conjugate can be used to detect biotin in a signal amplification scheme in conjunction with chromogenic substrates or fluorogenic HRP substrates, such as the tyramide signal amplification (TSA) technology.

The streptavidin-AP, streptavidin-HRP, and streptavidin-FITC conjugates use the sensitive streptavidin-biotin interaction to allow flexible, rapid, and sensitive immuno-detection (Bayer and Wilchek, 1980; Guesdon et al., 1979).

Thermo Scientific Pierce High Sensitivity Streptavidin-HRP, 0.5 mL is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Streptavidin-HRP (M00091) is coupled streptavidin with horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated primary or secondary antibodies in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry, hybridoma screening systems.

Horseradish peroxidase (HRP) is a heme-containing enzyme. HRP-Streptavidin consists of the protein streptavidin, as a secondary detection reagent, conjugated to the enzyme HRP. The conjugate is used in ELISA to increase the detection of various kinds of targets.

Steptavidin is coupled to horseradish peroxidase (HRP) using citrate buffer, pH 6.0, and a modified published procedure.1 The HRP used for coupling is Catalog Number P8375, Type VI. This HRP contains predominantly the C isozyme (~75%), but includes all others as well, except for the most acidic isozymes.

Activated streptavidin (SA) and horseradish peroxidase (HRP) are covalently conjugated to a polymer backbone. Multiple active biomolecules on each polymer chain increase the biotin binding capacity and amplify the peroxidase enzyme signal.