The primary objective of the current investigation was to develop and validate a rapid, single-step separation technique for the complete removal of MEF feeder cells from mES cells. A discontinuous density gradient was prepared using Histopaque 1119 at incremental percentages from the top to bottom of a test tube (20, 40, 60, and 100% in ...

addition of Wnt1 CM to both MEF-1 and MEF-7 signifi cantly increased the number of undifferentiated colonies, while the addition of Sfrps promoted differentiation. Together, these results suggest that microenvironment, ECM, and soluble factors expressed by MEF-1 are signifi cantly better at maintaining self-renewal and pluripo-tency of hESCs.

2012年8月8日 · Conventional procedures of these processes require co-culture with primary mouse embryonic fibroblasts (MEFs) to support self-renewal and clonal growth of human iPSCs as well as embryonic stem cells (ESCs). However, the variability of MEF quality affects the efficiencies of all these steps.

Long-term cultures of human embryonic stem (hES) cells require a feeder layer for maintaining cells in an undifferentiated state and increasing karyotype stability. In routine hES cell culture, mouse embryonic fibroblast (MEF) feeders and animal component-containing media (FBS or serum replacement) …

2024年3月14日 · 差异相关性总和( sum of correlation differ-ences, SCD) 测量融合图像与源图像的差异来表征融合算法优劣，D X,F 表示融合图像 F 与源图像 X 的差分图像。 SCD 越高,意味着融合图像 包含源图像中的信息越丰富

2009年3月24日 · A paracrine regulation was recently proposed in human embryonic stem cells (hESCs) grown in mouse embryonic fibroblast (MEF)-conditioned media (MEF-CM), where hESCs spontaneously differentiate...

2021年5月26日 · We report here simplified bESC culture conditions based on replacing custom base medium with a commercially available alternative and eliminating the need for MEF feeders by using a chemically ...

MEF2家族蛋白包括MEF2A、B、C、D，共享一个类似的N-末端，含有一个高度保守的MADS-box结构域和一个紧邻MEF2域。 MADS-box和MEF2域分别有55个和29个氨基酸，有助于DNA结合和二聚化。 在MEF2蛋白C-末端的反式激活域结构具有多样性。 MEF2B是其中最简单的蛋白。 MEF2A、C和D含有多种氨基酸和磷酸化的潜力。 MEF2的生理功能和信号转导通路. MEF2家族主要与各种生理过程相关，包括肌肉的形成、神经系统发育、心脏发育等。 它们也涉及人类疾 …

mef细胞分选自小鼠的胚胎，低温存 储之前，细胞经过存活率测试（冷冻复苏后的存活率>80%），并通过无菌、支原体检测。 MeilunCell ® 小鼠胚胎成纤维细胞取自怀孕13.5d 的KM 小鼠胚胎，作为ES 饲养层细胞使用

2024年1月18日 · 《科学引文数据库》 (Science Citation Database 简称：SCD)，是我国第1个涵盖自然科学、工程与技术、农林科学、医药科学、人文学科、社会科学等全部非保密学科的大型引文数据库。 SCD的应用领域之一就是作为 中国管理科学研究院 《中国大学评价》、《中国大学研究生院评价》课题源期刊数据库，用于评价中国普通本科高校和以创新为主的科研机构的群体创新能力。 SCD还可广泛应用于多个领域。 SCD的理论基础基于以下三个方面： 1、布拉德福 …