2014年10月16日 · To identify the subset of 24-h rhythmic proteins, JTK_CYCLE algorithm was used on the SCN proteomic (2112 proteins) or the time-of-day proteomic (421 proteins) dataset under R language. The JTK_CYCLE algorithm allows the user to input integer values when defining the (non-statistical) parameters of a search.

2024年10月2日 · Given the enrichment of ‘circadian rhythms signalling’ pathway, we explored whether chronic stress affects the rhythmicity of core clock genes in the SCN and Hb. To this end, we performed JTK...

2021年10月1日 · Signals from the central circadian pacemaker, the suprachiasmatic nucleus (SCN), must be decoded to generate daily rhythms in hormone release. Here, we hypothesized that the SCN entrains...

2015年11月2日 · To reveal the finer details of circadian variation in the mammalian SCN transcriptome we combined laser-capture microdissection (LCM) and RNA-seq over a 24 hr light / dark cycle. We show that 7-times more genes exhibited a classic sinusoidal expression signature than previously observed in the SCN.

2020年11月10日 · Analysis of rhythmic metabolites was performed using the nonparametric test JTK_CYCLE , incorporating a window of 20 to 28 h for the determination of circadian periodicity. A complete list of oscillating metabolites and JTK_CYCLE analysis is provided in Dataset S3 for the SCN and in Dataset S4 for the mPFC; see also circadiomics.ics.uci.edu .

哺乳动物视交叉上核（Suprachiasmatic Nucleus，SCN）可以在不同环境下产生强大的昼夜行为，但其潜在的神经机制尚不清楚。 2023年5月11日，中国科学院脑科学与智能技术卓越创新中心（神经科学研究所）严军研究组题…

2024年4月19日 · Moreover, our experiments in adult mouse reveal that TDP-43 knockdown, specifically within the suprachiasmatic nucleus (SCN), induces locomotor arrhythmia, arrhythmic c-Fos expression, and depression-like behavior. This observation offers valuable insights into the substantial impact of TDP-43 on the behavioral aberrations associated with ALS/FTD.

2023年11月13日 · As a dominant zeitgeber, feeding entrains extra-suprachiasmatic nucleus (SCN) clocks and drives metabolic and food anticipatory activity (FAA) rhythms 1. The precise circuitry underlying feeding...

2019年5月15日 · We performed a circadian RNA expression profile of the mammalian biological clock, the suprachiasmatic nucleus (SCN) in C57/BL6 mice, at 2-hour resolution using microarrays, and at 6-hour resolution using RNA-seq.

The suprachiasmatic nucleus (SCN) is the master daily pacemaker in mammals, responsible for driving daily rhythmic behaviour and physiology. By combining laser capture microdissection (LCM) and RNA-seq, we provide the first detailed examination of the mammalian SCN transcriptome over a 24-hour light / dark cycle. Methodology SCN RNA sample ...