SMI 312 visualizes axons in an area-specific maturation pattern in human fetal brain. The antibody cocktail defines nuclear borderlines and is useful in establishing early connectivity with SMI 311, anti-neurofilament (not phosphorylated) identified dendrites.

Optimal immunostaining with SMI 311 and SMI 312 is achieved when specimens are fixed in a mixture of paraformaldehyde and picric acid for up to 3 days and sections are incubated free-floating. Neurons, with their dendritic domains immunostained by SMI 311 in a Golgi-like manner, can be completely visualized in relatively thick sections.

2022年12月1日 · 左图：免疫荧光标记的阿尔茨海默病人脑中的神经纤维蛋白SMI-312； 右图：在5xFAD小鼠中通过腺病毒介导的在淀粉样沉积周围的神经束标记。 这一病变结构传统上称为营养不良性神经突(dystrophic neurites)，被认为是轴突损伤后的退行性结构。

SMI 311 provides an early marker of neuronal migration and differentiation in human fetal development yielding Golgi-like images without the disadvantages of lack of selectivity and poor specificity of the Golgi technique.

2024年12月9日 · SMI 312 visualizes aberrantly sprouting axons in neuritic plaques derived from cortico-cortical fibers in Alzheimers disease and identifies loss of synaptic circuitry proposed to be the basis of memory. This antibody is effective in immunoblotting, immunocytochemistry and …

visualizes axons in an area-specific maturation patternin human fetal brain; visualizes aberrantly sprouting axons in neuriticplaques derived from cortico-cortical fibers in A.D. and identifies loss of synaptic circuitry. No Available Comments. To make a comment you must login or register. No Available References. No Available Further Reading.

(G–I) Untransfected (G), Medium (H), and High B (I) clones differentiated for 7 days stained with antibody SMI-312 that stains neurofilament proteins, but predominantly NF-M. All cells showed SMI-312 positive neuronal processes consistent with differentiated morphology.

Neurofibromin (SMI-312) is recommended for detection of Neurofibromin of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunofluorescence and immunohistochemistry

312 is a convenient marker for axons in general. SMI 312 visualizes axons in an area-specific maturation pattern in human fetal brain. The antibody cocktail defines nuclear borderlines and is, thereby, useful in establishing early connectivities with SMI 311, antinonphosphoneurofilament-identified dendrites. SMI 312 visualizes