The results showed that Sp1 overexpression inhibited the expression and promoter activity of PTEN and correspondingly upregulated AKT phosphorylation, whereas Sp1 knockdown upregulated the expression and promoter ability of PTEN and downregulated AKT phosphorylation.

2022年4月14日 · Sp1 transcription factor (SP1) was predicted and identified as an upstream regulator of PTEN. SP1 suppressed PTEN transcription. Silencing of SP1 enhanced mouse survival and alleviated the lung injury, and it promoted the M1 polarization of macrophages in murine lung tissues.

Phosphatase and tensin homologue deleted on chromosome 10 (PTEN) is one of the most frequently disrupted tumor suppressors in cancer. The lipid phosphatase activity of PTEN antagonizes the phosphatidylinositol 3-kinase (PI3K)/AKT/mTOR pathway to repress tumor cell growth and survival.

2012年10月26日 · In this study, we examined (i) whether Sp1 could inhibit the expression and promoter activity of PTEN through Sp1-binding sites on the PTEN promoter, (ii) whether Sp1 acetylation and HDAC1 recruitment are responsible for the Sp1 inhibition of PTEN and (iii) whether Sp1 is involved in the development and aggressiveness of cancers.

2012年10月15日 · Through dephosphorylation of Sp1, PTEN regulates growth factors in cancer cells (Kang-Park et al., 2003, Pore et al., 2004) and regulators of cancer metastasis (Rankin et al., 2009, Sze et al., 2011). PTEN regulates Sp1 via suppression of downstream kinases, which leads to hypo-phosphorylation of Sp1 and suppresses Sp1 DNA binding.

2009年2月13日 · Studies using PTEN mutants show that its protein phosphatase activity directly modulates p75NTR protein expression. Nuclear relocalization of PTEN subsequent to LN stimulation suggests transcriptional control of p75NTR expression, which was confirmed following EMSA and ChIP analysis of Sp1 transcription factor binding activity.

2018年6月29日 · Highly expressed miR-150-3p suppressed the growth of glioma cells partially via targetting the SP1-PTEN signaling pathway. These findings provided novel insights into the functional mechanism of miR-150-3p in glioma.

本研究拟探索SP1能否通过介导miR-205/PTEN,活化Akt信号通路调控自噬，以减轻缺血再灌注急性肾损伤，从而为改善急性肾损伤提供新的视角和治疗靶标。 第一部分 SP1在缺血再灌注诱导的急性肾损伤体外模型中的作用 目的： 体外培养HK-2细胞，构建HK-2细胞缺氧-复氧损伤模型，探讨SP1、miR-205及PTEN在缺氧-复氧损伤HK-2细胞中的表达水平变化及探讨过表达SP1对缺氧-复氧损伤的HK-2细胞的作用和影响，同时观察上述过程中自噬相关蛋白、细胞增殖、细胞凋亡率 …

SP1 suppressed PTEN transcription. Silencing of SP1 enhanced mouse survival and alleviated the lung injury, and it promoted the M1 polarization of macrophages in murine lung tissues. However, further downregulation of PTEN increased protein kinase B (Akt) phosphorylation and blocked the alleviating roles of SP1 silencing in T2DM-PTB.

2012年10月26日 · The results showed that Sp1 overexpression inhibited the expression and promoter activity of PTEN and correspondingly up-regulated AKT phosphorylation, whereas Sp1 knockdown up-regulated the...