2019年12月27日 · Combining surface plasmon resonance (SPR) with atomic force microscopy (AFM), here we studied two complement system proteins at the single-molecule level: C3b, the proteolytically activated form of C3, and factor H (FH), the surface-sensing C3b-binding complement regulator.

2018年12月1日 · Here, we present the first use of a combined SPR-AFM approach to prepare and characterize membrane-mimetic surfaces with lipid raft-like domains, and we demonstrate the ability of AFM to determine the topological and nanomechanical properties of the membrane on a SPR sensor chip.

2023年1月29日 · Surface plasmon resonance (SPR) combined technology is a powerful tool for investigating interface phenomena. This paper reviews all combination technologies of SPR with various conventional detection systems, emphasizing the combination with electrochemistry, Raman spectroscopy, and mass spectrometry.

2006年8月30日 · The basic theory of SPR is provided to show the importance of the surface sensitive nature of this optical transduction technique. The present work suggests that both may prove both for SPR and other biosensing applications.

2019年1月3日 · The combination of not-attempted-before exotic sensing material and SPR architecture represents an approach to unlocking the ultrasensitive detection of miRNA and …

这项研究的目的是开发利用原子力显微镜（afm）和表面等离振子共振（spr）来研究受体与其配体之间的二价相互作用的键合化学。 我们构想了一个三臂结构，该结构由柔性链组成，该柔性链连接到大型刚性核，在末端带有正交的官能团，用于形成和连接（或固定 ...

2008年4月1日 · Atomic force microscopy (AFM) and surface plasmon resonance (SPR) are two important techniques to study the interaction between biomolecules under physiological conditions.

2018年12月1日 · The formation of a SLB on the SPR sensing surface in a reproducible way was assessed by the combined use of the SPR technique with AFM. The interaction of a cholesterol-extracting drug with SLBs was studied as a model of membrane-lipophilic biomolecule interaction.

The results of SPR and AFM studies are presented that have shown that each of these schemes promotes improved binding of various proteins. A comparative study into two novel and diverse schemes designed to improve immobilization of biomolecules for biosensing purposes is presented, suggesting that both may prove both for SPR and other ...

本项目拟通过体外原子力显微镜（AFM）力谱分析和表面等离子共振技术（SPR），从生物物理学和生化反应动力学角度直观的解析大肠杆菌和谷氨酸棒杆菌不同启动子与RNA聚合酶之间结合/解离过程中的交互作用,进而采集其结合力、结合概率、亲和度、结合/解离速度常数等基本参数,并利用主成分分析等策略关联其体内报告基因的转录及表达量，构建基于体外交互作用分析的启动子强度表征新策略，为不同强度的启动子元件设计、初步筛选提供高效的方法。 ，国自然项目查询 …