SSE1 | SGD - Saccharomyces Genome Database
2006年2月7日 · SSE1 and SSE2 are two of nine cytosolic forms of HSP70 found in S. cerevisiae (SSA1, SSA2, SSA3, SSA4, SSB1, SSB2, SSE1, SSE2, SSZ1).SSE is the yeast homolog of …
A first-in-class inhibitor of Hsp110 molecular chaperones of
2023年5月12日 · Previously, we have solved the first X-ray crystal structure of a full-length Hsp110 using Sse1, revealing extensive NBD-SBD contacts in the ATP-bound state 54. In addition, two structural...
Sse1, Hsp110 chaperone of yeast, controls the cellular fate during ...
2024年4月5日 · Sse1 is a cytosolic Hsp110 molecular chaperone of yeast, Saccharomyces cerevisiae. Its multifaceted roles in cellular protein homeostasis as a nucleotide exchange factor (NEF), as a protein-disaggregase and as a chaperone linked to …
Mutational Analysis of Sse1 (Hsp110) Suggests an Integral Role …
The yeast Hsp110 chaperone Sse1 is a conserved protein that is a noncanonical member of the Hsp70 protein superfamily. Sse1 influences the cellular response to heat stress and has also been implicated in playing a role in the propagation of prions in yeast.
Interdomain interactions dictate the function of the
2021年9月1日 · In this study, we carried out biochemical analysis on Msi3, the sole Hsp110 in Candida albicans, to dissect the unique allosteric coupling of Hsp110s using three mutations affecting the domain–domain interface. All the mutations abolished both the in vivo and in vitro functions of Msi3.
Early Steps of Protein Disaggregation by Hsp70 Chaperone and
2024年9月20日 · In vivo studies on yeast chaperones demonstrated that the Hsp110 co-chaperone Sse1 plays a key role in Hsp70 recruitment to aggregates and is essential for protein disaggregation (Kaimal et al, 2017).
Disease-associated mutations within the yeast DNAJB6 homolog
2022年8月5日 · Here, we show how mutations analogous to those found in LGMDD1 affect Sis1 (a functional homolog of human DNAJB6) function by altering the structure of client protein aggregates, interfering with...
酵母Hsp110 Sse1在功能上与Hsp70伴侣Ssa和Ssb相互作用 …
Ssa1是酵母交配信息素α因子到内质网的翻译后易位的必需条件。像ssa突变体一样，我们证明sse1delta细胞会积累prepro-alpha因子，但不会共翻译导入蛋白质Kar2，这表明Sse1和Ssa之间的相互作用在体内具有重要的功能。
Substrate binding by the yeast Hsp110 nucleotide exchange factor …
2017年5月24日 · Sse1 sbd retains nucleotide-binding and nucleotide exchange activities while exhibiting severe deficiencies in chaperone holdase activity for unfolded polypeptides. In contrast, we observed no effect of the SBD mutation in reconstituted disaggregation or refolding reactions in …
The yeast Hsp110 Sse1 functionally interacts with the Hsp70
2005年12月16日 · Sse1 stimulated Ssa1 ATPase activity synergistically with the co-chaperone Ydj1, and stimulation required complex formation. Ssa1 is required for post-translational translocation of the yeast mating pheromone alpha-factor into the endoplasmic reticulum.