Here, we have developed pH-insensitive fluorescent tools to monitor STIM1 activation and examined the impact of pH i changes on STIM1 function. Our findings reveal that, beyond its roles as an ER Ca 2+ sensor, STIM1 can sense fluctuations in cytosolic pH i through two conserved histidine residues.

Here, we explore the role of STIM1, anERcalcium(Ca2+) sensor mediating Store OperatedCa2+Entry (SOCE), in sensing pHi changes. Our study reveals that STIM1 functions as a sensor for pHi changes, independent of its Ca2+-binding state.

2011年4月17日 · Heating cells caused clustering of STIM1 at temperatures above 35 °C without depleting Ca 2+ stores and led to Orai1-mediated Ca 2+ influx as a heat off-response (response after cooling)....

Our results suggest that the phosphoinositides are PM components responsible for targeting STIM1 to ER–PM junctions and reveal a role for the phosphoinositides in the activity of SOCs.

2019年1月14日 · Here, we examined the phenotypes of STIM1-deficient cells and observed that loss of STIM1 induced spontaneous activation of the STING-TBK1-IRF3 pathway to activate type I IFN responses under...

2024年11月12日 · STIM1 胞浆区的两个保守组氨酸残基（ H355 和 H407 ）是 STIM1 的 pH i 感知功能的关键氨基酸残基。在 pH i 升高时， STIM1 活性增加增强 SOCE 活性，引发心肌肥大。这表明 STIM1 可以感知并响应 pH i 的变化，从而在 Ca 2+ 与 pH i 调控中起到关键作用。

2017年8月29日 · Here we investigate the intracellular pH (pH i) dependence of I CRAC mediated by Orai1 and STIM1ectopically expressed in HEK293 cells. The results indicate that pH i affects not only the amplitude of the current, but also Ca 2+ dependent gating of CRAC channels.

To measure the luminal pH of LEs in both control and ECs silenced for STIM1, we exploited the acetoxymethyl (AM) ester-modified fluorogenic intracellular pH probe pHrodo Green AM ( Kulkarni...

2024年12月1日 · Our findings reveal that, beyond its roles as an ER Ca 2+ sensor, STIM1 can sense fluctuations in cytosolic pH i through two conserved histidine residues. The release of H + from these residues activates STIM1, thereby enhancing STIM1-mediated SOCE and promoting cardiac hypertrophy.

2017年11月24日 · Here, we show that ablation of the store-operated-Ca 2+ -entry regulator STIM1 in mouse myeloid cells impairs cross-presentation and DC migration in vivo and in vitro. Stim1 ablation reduces Ca...