THE scanning tunnelling microscope (STM) has been used to visualize DNA 1 under water 2, under oil 3 and in air-, Images of single-stranded DNA...

1996年2月29日 · Abstract: The systematic removal or destruction of DNA by the scanning tunneling microscope (STM) stems from the low conductivity of the molecule. Imaging DNA at exceedingly low currents and high voltages prevents damaging contact in humid conditions.

Scanning tunneling microscopy (STM) is a technique that can be used to directly observe individual biomolecules at near-molecular scale. Within this framework, STM is of crucial significance because of its role in the structural analysis, the understanding the imaging formation, and the development of relative techniques.

Uncoated double-stranded DNA dissolved in a salt solution was deposited on graphite and imaged in air with the scanning tunneling microscope (STM). The resolution was such that the major and minor grooves could be distinguished. The pitch of the helix varied between 27 and 63 angstroms in the images obtained.

1981年，扫描隧道显微镜（Scanning Tunneling Microscope,STM）问世，由于其可以观察物质表面形貌，获得样品表面局域态密度，定位和操纵原子等，被公认为20世纪80年代世界十大科技成就之一。

2003年3月28日 · Single-molecule imaging by scanning tunnelling microscopy (STM) yields the atomic-resolution (0.6 Å) structure of individual B-type DNA molecules. The strong correlation between these STM structures and those predicted from the known base sequence indicates that sequencing of single DNA molecules using STM may be feasible.

1999年7月20日 · Here we report reproducible and reliable high-resolution STM images of DNA deposited on a well-defined Cu(111) substrate under UHV conditions using the pulse injection method. The STM images clearly exhibit the sub-molecular structures, i.e. double helix and individual bases, for the first time.

2009年7月5日 · Tanaka and Kawai have achieved partial sequencing of a single-stranded DNA molecule by using a combination of STM imaging and scanning tunnelling spectroscopy (STS) 5. The STM works by...

2004年1月8日 · 摘要： 用自制的stm,经高序定向热解石墨(hopg)对扫描组件进行定标后,采用展开法制样,在常温常压的自然条件下,对猪脾dna分子进行了观测研究.得到了石墨表面清晰的原子分辩图象以及猪脾dna分子双螺旋结构的stm形貌图.介绍了生物样品的制备方法,对实验结果进行 ...

1990年7月19日 · The scanning tunnelling microscope (STM) has been used to visualize DNA under water, under oil and in air. Images of single-stranded DNA have shown that submolecular resolution is possible. Here we describe atomic-resolution imaging of duplex DNA.