2022年12月27日 · Here, we report a new method MM-seq (modification-induced mismatch sequencing) for genome-wide 6mA mapping based on a novel detection principle. We found that modified DNA bases are prone to...

2024年9月14日 · Our study provides new insights into the development of B cells and the heterogeneity of plasma cells in MM and suggests that WNK1 is a potential therapeutic target for MM.

2022年11月15日 · Bortezomib, commonly used for MM treatment, works on both MM and TME cells, but innate and acquired resistance easily develop. By single-cell RNA sequencing (scRNA-seq), we investigated...

Next generation sequencing (NGS)-based FISH (seq-FISH) has demonstrated improved sensitivity and similar specificity relative to clinical FISH at detecting relevant chromosomal abnormalities in MM. 27 We sought to examine whether scRNA-seq could also be useful in confirming the transcriptional consequence of translocations or improve clinical ...

单细胞测序在多发性骨髓瘤 (mm) 中的应用尤其有益，因为 mm 是一种高度异质性的疾病，具有不受控制的浆细胞克隆扩增。 单细胞 RNA 测序 (scRNA-seq) 先前已被用于了解 MM 肿瘤和免疫群体中的这种造血恶性肿瘤（Ledergor G. 等人，2018 年，Zavidij，O. 等人，2020 年）。

2023年2月6日 · We developed a novel approach, MinimuMM-seq, which enables the detection of translocations and copy-number abnormalities through whole-genome sequencing of highly pure CTCs. Application to CTCs in a cohort of 51 patients, 24 with paired BM, was able to detect 100% of clinically reported BM biopsy events and could replace molecular cytogenetics ...

MMseqs2 (Many-against-Many sequence searching) is a software suite to search and cluster huge protein and nucleotide sequence sets. MMseqs2 is free and open source software implemented in C++ for Linux, MacOS, and (as beta version, via cygwin) Windows.

2023年11月2日 · One promising approach is single-cell RNA sequencing (scRNA-seq), which can elucidate the transcriptome heterogeneity of individual cells and reveal previously unknown cell types or states in complex tissues. In this review, we outlined the experimental workflow of scRNA-seq in MM, listed some commonly used scRNA-seq platforms and analytical tools.

2023年1月7日 · 该研究报道了一种基于新的检测原理的全基因组 6mA 检测新方法 MM-seq （Modification-induced Mismatch Sequencing），并利用该方法评估了 6mA 在细菌、绿藻和哺乳动物细胞中的基因组位置，揭示了人类不同细胞类型拥有非常有限的 6mA 位点，这些位点零星分布在不同的基因组定位上。 敲除 RNA m6A 甲基转移酶 METTL3 后，基因组 DNA 中可检测到的 6mA 位点进一步减少。 自从在真核生物 DNA 上重新发现 6mA 修饰以来，研究人员对其能否成 …

Here, we have analyzed single cell RNA-seq data and bulk gene profiles to reveal a novel signature associated with MM development. The scRNA-seq data from GSE118900 was used to profile the transcriptomes of cells from MM patients at different stages.