近年来,SESI-HRMS呼气代谢组学研究取得突破性进展 [1],首次在无损、直接分析条件下,成功获得个体呼气代谢表型 (>500种代谢物) [2],并在实验室研究阶段成功用于: (1)诊断慢性阻塞性肺病 [3]; (2)识别乳癌生物标志物 [4]; (3)表征个体生物钟 [5],为个体的疾病预警、治疗以及健康护理开启了新的无损诊断平台。 SESI-HRMS呼气代谢组学研究有望广泛应用于医学、环境、制药等重要重点学科领域,在科研、政府、产业界发挥巨大作用 (图1)。

More specifically, secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) enables the detection of a broad range of breath-derived compounds simultaneously and with high sensitivity. Together with its rapid and non-invasive nature, direct breath analysis by SESI-HRMS raised particular interest for clinical applications.

2025年3月5日 · In sampling and pretreatment processes, SESI-HRMS/MS offers significant advantages over UHPLC-ESI-HRMS/MS. SESI-HRMS allows for real-time analysis of breath samples without any pretreatment and analyses of 12 replicates of a single sample can be completed within 10 min. Moreover, breath is ideal for continuous monitoring studies due to its ...

2024年6月27日 · Breath analysis with secondary electrospray ionization (SESI) coupled to mass spectrometry (MS) is a sensitive method for breath metabolomics. To enable quantitative assessments using SESI-MS, a system was developed to introduce controlled amounts of gases into breath samples and carry out standard addition experiments.

2022年1月2日 · Amongst the various analytical techniques existing to investigate the volatilome, the online, non-invasive sampling nature of secondary electrospray ionization-high resolution mass spectrometry (SESI-HRMS) provides sensitive and robust VOC detection that makes SESI-HRMS ideal for untargeted and targeted studies [18].

2024年10月11日 · SESI-HRMS shows higher sensitivity for some compounds, needing GC–MS and LC-MS annotation. Optimizing fragmentation techniques is crucial for comprehensive analysis in this field. Secondary electrospray ionization coupled to high-resolution mass spectrometry (SESI-HRMS) is a powerful method for the analysis of exhaled breath in real time.

2022年2月17日 · 本研究中使用了名为 SuperSESI 的先进二次电喷雾电离 (SESI) 源，并直接连接到 Thermo Q-Exactive 高分辨率质谱仪 (HRMS)。 我们进行了一系列实验，以确定我们优化的 SESI-HRMS 平台是否可以通过对体外挥发组图进行采样来区分癌症类型。

2019年12月16日 · To analyze the volatilome in real-time, we have developed a Secondary Electro-Spray Ionization source optimized for Orbitrap HRMS. Here we present how it works, what type of molecules it can detect, and what applications it enables.

本研究使用了一种名为 SuperSESI 的高级二次电喷雾电离 (SESI) 源，并直接连接到 Thermo Q-Exactive 高分辨率质谱仪 (HRMS)。 我们进行了一系列实验，以确定我们优化的 SESI-HRMS 平台是否可以通过体外取样来区分癌症类型volatilome 配置文件。

2016年6月15日 · 呼气分析具有无损、灵敏、实时、快速等技术优势。 近年来，呼气代谢组学研宄取得突破性进展，首次在无损、直接分析条件下，成功获得个体呼气代谢表型（种代谢物）并在实验室研宄阶段成功用于...