sfGFP激发发射波长是多少nm？sfGFP和GFP的区别？ - XEPU
2023年12月27日 · Superfolder GFP超折叠绿色荧光蛋白，简称sfGFP，是2005年发表的一种碱性（组成型荧光）绿色荧光蛋白。 sfGFP来源于Aequorea victoria，分子量约为26.8kDa。 …
Superfolder GFP :: Fluorescent Protein Database
2022年11月6日 · Superfolder GFP is a basic (constitutively fluorescent) green fluorescent protein published in 2005, derived from Aequorea victoria. It is reported to be a very rapidly-maturing …
Expression and use of superfolder green fluorescent protein at …
Superfolder GFP (sGFP) is a variant of the Green Fluorescent Protein that folds efficiently when fused to poorly folded proteins. In this study, we show that sGFP, but not enhanced GFP, is …
妙啊！没有＂火眼金睛＂也可以看见的遗传转化！ - 知乎
将其突变形式的sGFP（S65T）作为报告基因，在转基因烟草筛选中，只需在荧光显微镜下直接检测烟草植株的叶片，即可轻松筛选出转基因植株。如图所示，未转化的烟草叶片呈现鲜红色（ …
GFP及其衍生型荧光蛋白、突变体的详细介绍 - 知乎
近年来出现的新型wtGFP基因突变体的激发和发射谱发生了改变，热稳定性和荧光强度得到了提高，GFP报告基因在小鼠中的应用就是以这些变体作为基础的。 （4）增强型绿色荧光蛋 …
超级文件夹绿色荧光蛋白在高温体内的表达和使用：研究极端嗜热 …
超级文件夹GFP（sGFP）是绿色荧光蛋白的一种变体，当与折叠不良的蛋白融合时可以有效折叠。在这项研究中，我们显示sGFP，而不是增强的GFP，在极端嗜热菌Thermus …
Development and Applications of Superfolder and Split …
Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and …
Green fluorescent protein as a marker for expression of a
The sGFP gene is a synthetic human codon-optimized GFP gene with an S65T mutation in the chromophore, which leads to altered spectral qualities 13, 14. The S65T mutation and the …
superfolder GFP Sequence and Map - SnapGene
GFP variant that folds robustly even when fused to poorly folded proteins. Sequence Author: Waldo Lab. Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More …
SGFP2 :: Fluorescent Protein Database
SGFP2 is a basic (constitutively fluorescent) green fluorescent protein published in 2007, derived from Aequorea victoria. It has moderate acid sensitivity.
- 某些结果已被删除