常规方法采用限制性内切酶SmaI来验证ITR区域的完整性，但当缺失片段很小时，靠着凝胶电泳是无法区分的。 酶切法不能检测到ITR区域的小片段丢失。 (A)AAV质粒限制酶酶切位点图。

2025年2月18日 · The GENEWIZ AAV-ITR sequencing method is sufficiently robust to read through both wild-type and mutant ITRs, enabling detection of very challenging ITR truncations in clonal and heterogeneous populations of plasmid DNA.

基因组的两端是两个t形反向末端重复序列(itr)，这两个itr是aav的dna自我复制的起点以及触发病毒包装的信号，在病毒的复制和包装过程中起着关键作用，并且参与了病毒基因组在宿主基因组上的整合和逃逸过程。

2023年8月23日 · SmaI, XmaI or SrfI: There are commonly used for screening ITR deletions, which target the C-C’ arm of the ITR sequence, which can provide insight into potential deletions. MscI: It targets the terminal resolution site of ITR. AhdI: It …

倒位重复序列（ITR, Inverted Terminal Repeat）是指一段在基因组两端重复的核苷酸序列，但它们的方向是相反的。 ITR通常存在于一些病毒基因组中，如腺相关病毒（AAV）的单链DNA基因组中，起到关键的结构和功能作用。

Here we report an improved and universal SmaI qPCR method, based on cleaving all ITRs in AAV2 genome by SmaI with several advantages: (1) impact of all ITRs in ssAAV2 and scAAV2 was dismissed; (2) titers increased remarkably, up to 7-fold, especially for scAAV2; (3) the variation of titers was reduced when different primers were applied.

Restriction digestions have traditionally been performed to screen for ITR deletions. The enzymes SmaI and SrfI are often used, as they cut within the C-C’ arm of the ITR3. Given the limited resolution of gel electrophoresis, diagnostic digests fail to detect small deletions and point mutations outside the restriction site (Figure 4).

2023年10月19日 · ITRs are symmetrical 145-nucleotide sequences that flank the ends of the single stranded DNA genome of AAV in the native virus. In an AAV vector plasmid, they flank the gene of interest. A single ITR consists of palindromic arms (A-A’, B-B’, and C-C’) that give the feature its characteristic T-shape.

2022年5月26日 · aav 基因组的两端包含两个 145bp 的反向末端重复序列 (itr)，这会形成一种高度稳定的 t 型发夹结构，它在病毒的复制和包装过程中起到了关键的作用。然而这种特殊结构对于 aav 质粒生产和 qc 过程产生了不小的挑战。

Inverted terminal repeat (ITR): key element of an Adeno-associated virus (AAV) plasmid that is essential for the integrity of AAV transfer plasmid. Each AAV plasmid has 2 ITR sequences (145 bases each) at each end flanking the transgene, forming a palindromic