5 天之前 · 上述研究阐释了arf蛋白在囊泡分裂过程中的分子机制，填补了最后阶段囊泡分裂的关键机制信息缺失，为细胞内物质运输的调控研究提供了新思路。 3月21日，相关研究成果以 Structural elucidation of how ARF small GTPases induce membrane tubulation for vesicle fission …

The small ADP ribosylation factor (Arf) GTP-binding proteins are major regulators of vesicle biogenesis in intracellular traffic. [1] They are the founding members of a growing family that includes Arl (Arf-like), Arp (Arf-related proteins) and the remotely related Sar (Secretion-associated and Ras-related) proteins.

The ARF small GTPases play central roles in these mechanistic steps for multiple intracellular pathways. A molecular understanding of how ARF proteins act in coat recruitment and vesicle budding is being achieved through structural studies, but a similar level of understanding of its role in vesicle fission has remained elusive. ...

2006年5月1日 · The ADP-ribosylation factor (ARF) small GTPases regulate vesicular traffic and organelle structure by recruiting coat proteins, regulating phospholipid metabolism...

4 天之前 · ADP-Ribosylation Factor (ARF) small GTPases have been found to act in vesicle fission through a direct ability to tubulate membrane. We have pursued cryoelectron microscopy (EM) to reveal at 3.9 Å resolution how ARF6 assembles into a protein lattice on tubulated membrane. Molecular dynamics simulation studies confirm and extend the cryo-EM ...

2024年12月10日 · Here, we report that the ARF-like GTPase TITAN5 (TTN5) interacts with the large cytoplasmic variable region and protein-regulatory platform of IRT1. Heterozygous ttn5-1 plants can display reduced root iron reductase activity. This …

当对ARF晶格中蛋白结合界面的关键残基引入突变时，虽然ARF1与包被蛋白复合物（coatomer）的结合未受影响，但囊泡的分裂过程却明显受阻。 这一结果印证了此前的研究，即ARF1二聚体对囊泡分裂是必要的，且该二聚体的界面恰好位于螺旋晶格的四聚体界面区域。

2014年1月1日 · Small GTP-binding proteins of the ADP-ribosylation factor (Arf) family are key components of trafficking vesicles. In the past three decades a number of vesicular carriers, whose formation depends on members of the Arf family were identified, and general molecular mechanisms how these transport carriers form and operate were established.

Here, this review particularly focuses on the self-assembly processes of Rab- and Arf-family small GTPases in intracellular membrane trafficking and thus summarizes the current experimental evidence for self-assemblies of Rab and Arf proteins on lipid bilayers in an in vitro reconstitution system (Figs. 1 and 2; Mima 2018; Segawa et al. 2019 ...

2025年3月17日 · These include Arf members (Arl1, Arf1), Arf GEFs (Sed4, Sec7), other classes of organelle dynamics regulators such as Rab GTPases (Ypt31, Sec4), and proteins involved in membrane lipid metabolism ...