2023年8月18日 · Smo activation requires two mechanisms: first, Smo needs to be translocated to primary cilia, a microtubule-based organelle (Fig. 3); second, Smo localized to cilia undergoes...

2022年8月1日 · Cholesterol and PI4P may help SMO to anchor to the ciliary membrane. The Smoothened receptor (SMO, a 7 pass transmembrane domain, Class F GPCR family protein) plays a crucial role in the Hedgehog (HH) signaling pathway, which is involved in embryonic development and is implicated in various types of cancer throughout the animal kingdom.

2005年10月13日 · Here we show that mammalian Smoothened (Smo), a seven-transmembrane protein essential for Hh signalling, is expressed on the primary cilium. This ciliary expression is regulated by Hh pathway activity; Sonic hedgehog or activating mutations in Smo promote ciliary localization, whereas the Smo antagonist cyclopamine inhibits ciliary localization.

2022年8月1日 · The Smoothened receptor (SMO, a 7 pass transmembrane domain, Class F GPCR family protein) plays a crucial role in the Hedgehog (HH) signaling pathway, which is involved in embryonic development and is implicated in various types of cancer throughout the animal kingdom.

2005年8月31日 · Here we show that mammalian Smoothened (Smo), a seven-transmembrane protein essential for Hh signalling 3, is expressed on the primary cilium. This ciliary expression is regulated...

2012年8月3日 · We developed a microscopy-based high throughput screen for small molecules that inhibit ciliogenesis or Smo localization to cilia using IMCD3 cells stably expressing Smo-YFP, selected for their ability to produce long cilia. Because overexpression of Smo causes constitutive ciliary localization, the IMCD3 cell line exhibited constitutive ...

2015年11月23日 · We present a method that readily captures rapid snapshots of the ciliary proteome by selectively biotinylating ciliary proteins using a cilia-targeted proximity labeling enzyme (cilia-APEX). Besides identifying known ciliary proteins, cilia-APEX uncovered several ciliary signaling molecules.

2019年4月4日 · After introducing how cilia establish their unique proteome and lipidome, we examine the mechanisms that regulate protein entry into and exit out of cilia and how they dynamically control...

2008年6月27日 · In mammalian cells, Smo-dependent signaling requires translocation to primary cilia. We demonstrated that beta-arrestins mediate the activity-dependent interaction of Smo and the kinesin motor protein Kif3A.

2009年11月2日 · Surface Smo enters cilia before intracellular Smo in response to Shh. Antibodies can alter the trafficking behavior of proteins, so we used a completely independent method to label Smo and follow its transport.