2021年12月15日 · SpeI has a High Fidelity version SpeI-HF ® (NEB #R3133). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.

Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and …

BspEI is an isoschizomer of BspMII. Blocked by overlapping dam methylation and impaired by CpG methylation. Is BspEI activity sensitive to dam, dcm or mammalian CpG methylation? How many base pairs should be added at the end of a PCR primer next to the BspEI recognition site to guarantee that BspEI will cut properly?

Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type. Learn why NEB restriction enzymes are the trusted industry standard.

Thermo Scientific BcuI (SpeI) 限制性酶识别 A^CTAGT 位点，并在 37°C 的 Tango 缓冲液中切割效果最佳（同裂酶：AhlI、SpeI）。 Thermo Scientific 常规限制性内切酶是一大类高质量的限制性酶，浓度为 10 U/μL。

2020年11月3日 · 2.双酶切的问题 3.酶切底物DNA，切不开 4.DNA经酶切后，电泳无带或出现smear现象 5.甲基化酶

本酶提供高保真版 SpeI-HF ® （NEB #R3133）。 高保真（HF）限制性内切酶在 rCutSmart 缓冲液中具有 100% 活性；统一缓冲液意味着更加直接、简化的样品处理过程。 HF 内切酶还会显著降低星号活性。

本酶提供高保真版 SpeI-HF ® （NEB #R3133）。 高保真（HF）限制性内切酶在 rCutSmart 缓冲液中具有 100% 活性；统一缓冲液意味着更加直接、简化的样品处理过程。 HF 内切酶还会显著降低星号活性。

通过毛细管电泳和峰值分析确定降解百分比。 分辨率达到 单核苷酸水平。 产品来源 大肠杆菌菌株，携带有克隆自球衣菌属（Sphaerotilus）（ATCC 13923）并经修饰的 SpeI 基因 产品类别: S 字母开头的限制性内切酶产品, High-Fidelity (HF®) Restriction Endonucleases Products,

View Genome for SpeI... DNA RNA hybrids... RNA duplexes... Single stranded DNA... Combined report... Methylation Sensitivity...