The phosphoprotein synapsin I (Syn I) plays a crucial role during axonogenesis and synaptogenesis as well as in synaptic transmission and plasticity of mature neurons. Abnormalities in SYN1 gene expression have been linked to important neuropsychiatric disorders, such as epilepsy and autism.

2022年6月28日 · Optogenetically modified SH-SY5Y cells were highly viable (>85%) after lentiviral transduction and neuronal differentiation. The cells demonstrated properties of functional neurons, developing beta III tubulin (TuJ1)-positive long neurites, forming neural networks, and expressing vGlut2. Action potentials were produced upon optical stimulation.

2022年6月7日 · We set out to characterise and compare the proteomes of undifferentiated and differentiated SH-SY5Y cells. For this, we explore neuronal differentiation following standard protocols using (i) RA...

2024年11月25日 · Our approach demonstrates the strong expression of glutamatergic neuron phenotypes in 3D SH-SY5Y neural spheroids, offering a promising tool for high-throughput NDD modeling and advancing drug ...

2020年8月31日 · SH-SY5Y cells, with inducible expression of human α-Syn (under the control of Tet-off response element), were used to investigate the potency of exogenously applied recombinant α-syn PFFs (Figure S1a) to seed endogenous α-Syn into fibrillar aggregated species.

2024年10月22日 · 使用免疫荧光检测sh-sy5y细胞中突触相关蛋白的表达 模型组和H89组的红色荧光显著降低，表明Syt1、Psd95和Syn1蛋白的表达显著降低（P<0.05）。 在其他组中，含补阳还五汤的血清或福司柯林处理后，Syt1、Psd95和Syn1的荧光强度增加（P<0.05）。

2013年2月1日 · D and E, chromatin immunoprecipitation of human (D) and mouse (E) SYN1 promoters was performed from SH-SY5Y and N2a cells, respectively. The PCR-amplified regions are boxed in the top panel (human, −780 to −280; mouse −520 to −256). Sp1 binding was revealed using 2 μg of anti-Sp1 antibody.

2022年9月1日 · The SH-SY5Y human neuroblastoma cell line (ATCC® CRL-2266™) is one of the most used cell lines in neurosciences, either undifferentiated or differentiated into neuron-like cells.

Results: Sp1 binds to the SYN1 promoter, activating its transcription. Conclusion: Sp1 is a novel regulator of SYN1 transcription, whose activity is inhibited by REST and CpG methylation. Significance: Elucidating the mechanisms underlying basal activation of neuron-specific genes is fundamental to understand

When probes were incubated with nuclear extracts from human SH-SY5Y or murine N2a cells, the formation of slower migrating DNA-protein complexes was observed (Fig. 1, B and C , lane 1 ). The...