There are several steps to prepare the sample using the Nextera XT protocol. At some points we use TCY plate, at other points we use MIDI plate. Also when you seal the plate with the microseal,...

If you do not already have a WG#-DNA plate, create one by adding DNA into either a: • MIDI plate: 20 μl to each WG#-DNA plate well • TCY plate: 10 μl to each WG#-DNA plate well Apply a barcode label to the new WG#-DNA plate. [ ] 2. At the robot PC, select MSA3 Tasks | Make MSA3. Make MSA3 Transfer DNA samples into the MSA3 plate. Denature ...

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Apply a BCD barcode label to a new 0.8 ml MIDI plate or a new 0.2 ml TCY plate. Transfer the BCD to the plate. Use the volume appropriate for your plate: The example illustrates 96 samples. For 48 samples, fill only the blue side of the plate. If you are stopping, heat-seal the plate, and store at -25°C to -15°C for up to 30 days.

TCY plates, 1 per run • ... EX automation uses only MIDI plate. Skirted PCR microplates, 96-well, 200 µl . Bio-Rad, catalog # HSP-9601. PGx amplification plate, Infinium Pharmacogenomics protocol only. Storage microplates, 96-well, 0.8 ml. MIDI plates, 1 per run . Thermo Fisher Scientific, catalog # AB0765.

This protocol explains how to prepare up to 96 indexed paired-end libraries, followed by enrichment using whole exome or custom probe panels, and subsequent cluster generation and DNA sequencing using the reagents provided in the …

To sequence whole genome microbial DNA isolated from animal fecal matter and perform functional analysis of bacterial communities. This protocol was adapted from: Nexteraâ XT DNA Sample Preparation Guide. (2012). Illumina. Label a new 96-well TCY plate NTA (Nextera XT Tagment Amplicon Plate).

Illumina FastTrack Service allows customers to access cutting-edge Illumina products without the need to set up a dedicated genotyping laboratory. FTS customers can conduct whole-genome association studies, DNA copy number studies, linkage analysis, fine mapping, and whole genome DNA methylation studies—all in a cost-effective and timely manner.

How does the Nextera XT Assay Work? The Nextera XT DNA Sample Preparation Kit uses an engineered transposome to simultaneously fragment and tag ("tagment") input DNA, adding …

2014年6月1日 · To assess plate-to-plate variation, a peptone titration study was performed in three 24DW plates with CHO line 5 as described in Materials and Methods. Each plate contained six different concentrations of TCY peptone in duplicate wells.