Nov 17, 2021 · Here we developed chemical strategies to mimic the reaction intermediates of the first and second ubiquitin transfer steps, and determined the cryo-electron microscopy structures of Ubr1 in...

泛素连接酶E3 成分N- 识别蛋白1 （ubiquitin protein ligase E3 component N-recognin 1，Ubr1）已被证明是N端规则信号通路最重要的E3 连接酶，能识别含不稳定氨基酸末端的蛋白质[7]，结 构上存在3 个底物结合位点，分别能特异性结合蛋 白质的碱性N端残基，大的疏水性N 端残基，以及 分子内包含Ubr1...

Sep 11, 1998 · The rfa1td plasmid was linearized with Bcl I and transformed into the yeast strains SX46A and SX46A ubr1:: his3. Recombinants of ura3 + were selected and integration of the rfa1td allele into the genome was confirmed by Southern analysis.

Nov 14, 2010 · Substrates of the N-end rule pathway are recognized by the Ubr1 E3 ubiquitin ligase through their destabilizing amino-terminal residues. Our previous work showed that the Ubr1 E3 and the Ufd4...

Feb 7, 2025 · In this study, we take an in vitro reconstitution approach to understand Ubr1 reprograming by Roq1. We show that Roq1 differentially modulates the ubiquitination of distinct types of Ubr1 substrates. We then define two cooperating sequence motifs in Roq1 that are needed for binding and regulating Ubr1.

Apr 28, 2009 · Degradation is dependent on the E3 protein, which is encoded by the ubiquitin‐protein ligase gene UBR1 (Figure 2A), indicating proteasomal degradation by the N‐end rule pathway (Bartel et al, 1990). Furthermore, repression of pTEV expression rapidly restores protein levels of the target protein (Figure 2B).

目的 寻找并验证Ubr1泛素化底物蛋白大规模筛选方法的可行性.方法 通过对啤酒酵母RJD347 (Ubr1野生型)和AVY26 (Ubr1缺失型)的差异蛋白质组学比较,分析酵母中可能存在的Ubr1直接或间接作用的底物蛋白.进一步通过外源表达实验验证差异蛋白与Ubr1之间的相关性.结果 组 ...

We show here that the type-1/2 sites of UBR1, the sole N-recognin of the yeast Saccharomyces cerevisiae, are located in the first ∼700 residues of the 1,950-residue UBR1. These sites are distinct in that they can be selectively inactivated by mutations, identified through a …

Apr 12, 2018 · Here we found that the C. elegans UBR-1 regulates glutamate level. When UBR-1 is defective, C. elegans exhibits increased glutamate; this leads to synchronization of motor neuron activity, hence defective locomotion when animals reach adulthood. UBR1-mediated glutamate metabolism may contribute to the physiological defects of UBR1 mutations.

May 12, 2010 · 我们的研究结果基于该质量控制系统的效率，表明细胞会利用生长潜力来避免与未折叠或错误折叠蛋白质积累相关的潜在毒性。 因此，Ubr1 和 Ubr2 代表了在细胞溶质核糖体上合成的蛋白质的新型质量控制途径的 E3 组分。

Proteins targeted for degra-dation are bound on their N-terminal residue by Ubr1 (also designated E3. a and N-recognin), which catalyzes the covalent attachment of ubiquitin to the protein substrate. Two zinc finger domains and the RING-H2 inger domain of Ubr1 are essential for substrate recognition.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26069-1-AP (UBR1 Antibody) at dilution of 1:200 (under 40x lens). The Proteintech guarantee covers …

2023年6月2日，北京大学生命科学学院朱健/刘敏课题组在 Journal of Cachexia, Sarcopenia and Muscle 杂志发表了题为 “Identification of Ubr1 as an amino acid sensor of steatosis in liver and muscle” 的研究论文，发现E3泛素连接酶Ubr1是肝脏和肌肉中重要的营养感应器。

Oct 23, 2020 · Ubr1 is a conserved ubiquitin ligase involved in the degradation of aberrant proteins in eukaryotic cells. The human enzyme is found mutated in patients with Johanson-Blizzard syndrome. We...

Feb 21, 2025 · We found that the functional loss of an evolutionarily conserved E3 ubiquitin ligase, UBR-1, leads to IVM resistance in Caenorhabditis elegans. Multiple IVM-inhibiting activities, including viability, body size, pharyngeal pumping, and locomotion, were significantly ameliorated in various ubr-1 mutants.

该文通过蛋白化学合成技术和冷冻电镜技术，开发了针对泛素E3酶的“化学捕获”策略，并揭示了泛素E3连接酶Ubr1识别并以K48链型多聚泛素化修饰底物的动态机制。

Oct 15, 2023 · 神经系统中兴奋/抑制（E/I）平衡对于调节大脑皮层信息流至关重要。 为了防止神经元过度活跃或静息，维持神经回路兴奋性和抑制性之间的动态平衡必不可少。 E/I失衡会导致多种神经发育和神经系统疾病,例如癫痫、自闭症和帕金森等。 本网站所转载的文章，均来自互联网，旨在传递更多信息。 鉴于互联网的开放性和文章创作的复杂性，我们无法保证所转载的所有文章均已获得原作者的明确授权。 如果您是原作者或拥有相关权益，请与我们联系，我们将立即 …

Sep 13, 2011 · Ubr1 is a conserved ubiquitin ligase involved in the degradation of aberrant proteins in eukaryotic cells. The human enzyme is found mutated in patients with Johanson-Blizzard syndrome.

Oct 9, 2023 · An alternative and complementary approach to address JBS is through the characterization of genetic modifiers of phenotypes exhibited by an UBR1 animal model. This approach is physiologi-cally relevant as it can reveal dys-regulated signaling pathways in the absence of UBR1, along with genetic suppressors of altered behaviors (Kelly & Stanley ...

Nov 27, 2024 · Proteomics analysis of the hypothalamus identified UBR1 as a potential E3 (ubiquitin protein ligase) ligase promoting ACE2 ubiquitination. Enhanced UBR1 expression, associated with ACE2 reduction, was confirmed in various tissues from hypertensive male mice and human samples.