2020年3月15日 · In the current study, we identified TRIM25, an E3 ubiquitin ligase, as a novel positive regulator in mediating NF-κB activation in human embryonic kidney 293T (HEK293T), HeLa cells, THP-1 cells, and PBMCs.

2019年8月5日 · In this study, we show that this antibody can specifically suppress tmTNF-α shedding by competing with a TNF-α converting enzyme that cleaves the tmTNF-α ectodomain to release sTNF-α.

2020年3月15日 · In the current study, we identified TRIM25, an E3 ubiquitin ligase, as a novel positive regulator in mediating NF-κB activation in human embryonic kidney 293T (HEK293T), HeLa cells, THP-1 cells, and PBMCs. The expression of TRIM25 promoted TNF-α–induced NF-κB signaling, whereas the knockdown had the opposite effect.

2011年2月18日 · TNF α induces association of USP4 with TAK1 to deubiquitinate TAK1 and downregulate TAK1-mediated NF- κ B activation. Overexpression of USP4 wild type, but not deuibiquitinase-deficient C311A...

Therefore, the present study investigated the role of the NF-κB transcription factor binding site in the transcriptional regulation of the CSE gene in 293T cells following treatment with TNFα using luciferase assays, as well as using western blotting and reverse transcription-quantitative PCR to examine the effect of TNFα on CSE expression in HU...

Therefore, the present study investigated the role of the NF-κB transcription factor binding site in the transcriptional regulation of the CSE gene in 293T cells following treatment with TNFα using luciferase assays, as well as using western blotting and reverse transcription-quantitative PCR to examine the effect of TNFα on CSE expression ...

2013年2月21日 · We investigated the role of EVER2 in death receptor-mediated apoptosis in EVER2-expressing human embryonic kidney 293 T (HEK-293T) cells and in EVER2 -silenced Jurkat cells, focusing on the TNF- α -induced survival/death decision.

在目前的研究中，我们确定了 TRIM25，一种 E3 泛素连接酶，作为介导人胚胎肾 293T (HEK293T)、HeLa 细胞、THP-1 细胞和 PBMC 中 NF-κB 活化的新型正调节因子。 TRIM25 的表达促进了 TNF-α 诱导的 NF-κB 信号传导，而敲低具有相反的效果。

HEK-Blue™ TNF-α cells were engineered from the human embryonic kidney HEK293 cell line to detect bioactive human and murine tumor necrosis factor-alpha (TNF-α) by monitoring the activation of the NF-κB and AP-1 pathways. In addition, these cells can be used for screening antibodies or small molecule inhibitors targeting the TNF-α pathway.

Tumor Necrosis Factor-α (TNF-α) is a potent pro-inflammatory cytokine that plays a role in the rheumatoid arthritis pathology, [3] psoriatic arthritis (PsA) and psoriasis. [15] It stimulates interleukins IL-1 and IL-6. Polymorphism in the TNF-α gene is associated with destructive arthropathy in PsA. [15]