2024年10月11日 · 该研究在国际上率先 开发了一种基于单分子长读段测序平台 （a single-molecule sequencing platform， SMS） 、在单细胞分辨率研究蛋白质-DNA相互作用的新方法，称为scNanoSeq-CUT&Tag。 使用scNanoSeq-CUT&Tag技术，该研究对体外培养的六种有代表性的人类细胞系和小鼠体内的外周血单核细胞以及睾丸组织进行了系统分析，结果证明该方法可以精准解析单个细胞内染色质修饰等表观遗传信息，包括组蛋白共价修饰以及转录因子在基因组中的 …

CUT&Tag（Cleavage Under Targets and Tagmentation）是Steven Henikoff团队于2019年基于CUT&RUN（ 一文搞懂CUT&RUN）进行改进的研究蛋白质与DNA相互作用的技术，该技术利用Tn5转座酶的特点，在切割染…

TnsC is the key regulator of transposition, interacting with both the TnsAB transposase and TnsD- attTn7. We show here that TnsC interacts directly with TnsB, and identify the specific region of TnsC involved in the TnsB–TnsC interaction during transposition.

2023年2月14日 · Here, we first applied biotinylated Tn5 transposase-mediated CUT&Tag (B-CUT&Tag) to produce high-quality libraries for interrogating TF-DNA interactions. B-CUT&Tag combines streptavidin-biotin-based DNA purification with routine CUT&Tag, optimizing the removal of large amounts of intact chromatin not targeted by specific TFs.

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TNS2 cut-off value for the ELISA test was 183.96 (ng/ml), with 76.9% sensitivity and 78.9% specificity. The TNS2 cut-off value for the PCR method was found to be 0.33, at which 63% sensitivity...

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2017年2月9日 · In order to get the WNS and TNS I’m searching for these values from the vivado.log file. I couldn’t find a better way to get TNS. Its actually pretty fast and much quicker than running get_timing_paths. After running each phys_opt_design command, I check if the WNS>=0.000 or if the TNS hasn’t improved from the previous phys_opt_design.

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