The genomic DNA and T-cell receptor excision circles (TREC) in the cell lysates are quantified in the real-time PCR assay, in triplicate, by using a fluorescent probe specific for the T-cell receptor delta-deletion TREC signal joint and a distinct fluorescent probe for the reference gene, albumin.

Newborn screening (NBS) by quantifying T cell receptor excision circles (TRECs) in neonatal dried blood spots (DBS) enables early diagnosis of severe combined immunodeficiency disease (SCID). In recent years, different screening algorithms for …

T-cell receptor excision circles (TRECs) are small circles of DNA created in T-cells during their passage through the thymus as they rearrange their TCR genes. [1] . Their presence indicates maturation of T cells; TRECs are reduced in SCID (severe combined immunodeficiency disease).

T细胞受体重排删除环TREC (T cell receptor rearrangement excision circle),是naive T细胞的特异性标志,是TCR基因重排后删除掉的环状DNA片段,在细胞内不能复制也不随细胞分裂而增殖,稳定地存在于细胞内并随着细胞的分裂不断被稀释.研究认为对TREC的定量检测可以直接评估胸腺的输出功能.本文将针对T细胞受体重排删除环的形成原理以及TREC定量检测方法、正常人TREC水平数据库的确立以及TREC定量检测的缺陷进行综合性论述.

2018年6月26日 · By measuring the copy numbers of T cell receptor excision circles (TRECs) and κ-deleting excision circles (KRECs), it is possible to identify T and B lymphopenia related disorders (including SCID, X-linked agammaglobulinemia (XLA) and ataxia telangiectasia (AT)).

2022年7月12日 · Test Indications: This assay is useful for evaluating thymic function in patients with cellular or combined primary immunodeficiencies, or receiving immunotherapy or cancer vaccines.

Severe combined immunodeficiency (SCID) is a life-threatening condition of newborns and infants caused by defects in genes involved in T cell development. Newborn screening (NBS) for SCID using the T cell receptor excision circle (TREC) assay began ...

A qPCR assay for KRECs can be multiplexed with the TREC assay, as demonstrated by several pilot studies and adoption of TREC/KREC screening in Israel, Sweden, Iran and Switzerland. 59-62 KREC screening could also detect primary immunodeficiencies affecting B cells at birth.

The T-cell receptor excision circle (TREC) assay is an effective screening tool for severe combined immunodeficiency (SCID). The TREC assay was designed to detect typical SCID and leaky SCID, but any condition causing low naïve T-cell counts will also be detected.

Results: The multiplex qPCR TREC assay showed nearly 100% amplification efficiency for each of the TREC and reference sequences, clinical validity for multiple forms of SCID, and an analytic limit of detection consistent with prevention of contamination.