The Fh8 is ranked among the best solubility enhancer tags as Trx, MBP, or NusA (Costa et al., 2013a), and it offers a specific and simple purification of the target proteins by using its natural …

We demonstrate the use of thioredoxin-1 as a fusion tag for carrier-driven crystallization and report the first structure of a protein fused to E. coli thioredoxin-1 (Trx). We chose Trx mainly …

硫氧还蛋白 （Thioredoxin,TrxA或Trx1）是存在于真核和原核生物体内的氧化还原酶，由α螺旋和β折叠连接组成，热稳定性极高，在植物的免疫系统中发挥重要的抗氧化作用。 1964 …

2020年1月28日 · Multiple comparisons of various protein fusion tags, such as Trx, SUMO, GST, MBP, NusA, PDI, and PDIb′a′, with the target protein revealed MBP as one of the most …

Trx•Tag™ is a peptide sequence grafted onto the recombinant protein which could be removed by enterokinase. This protein tag is a sulubilization tag that could assist in the proper folding in …

2009年1月1日 · Solubility tags such as Trx or NusA can also be linked with affinity tags such as His-tags for efficient purification of the fusion protein. Other tags such as S-tags or FLAG-tags …

在一些情况下，表达水平也能决定标签的选择—可溶标签如甘露糖结合蛋白 (mannan-bindingprotein,MBP)、硫氧还原蛋白标签（thioredoxin,Trx)、N 利用质 A(N …

Plasmid pET32a-TRXtag from Dr. Pamela Bjorkman's lab contains the insert None - thioredoxin is vector encoded and is published in Proc Natl Acad Sci U S A. 2002 Sep 3. 99 (18):11634-9. …

Thioredoxins (TRX) are small ubiquitous redox proteins of approximately 12 kD found in nearly all organisms and playing a key role in redox signaling and oxidative stress responses.

This unit describes a gene fusion expression system that uses thioredoxin, the product of the Escherichia coli trxA gene, as the fusion partner.