2017年12月1日 · Phosphorylated ribosomal protein S6 (pS6) is a commonly used marker for mTOR hyperactivation. In this study, immunohistochemical analysis was used to examine the presence of pS6 in the brains of 7 dpf WT, tsc2 +/− and tsc2 −/− larvae.

2021年1月8日 · Tuberous sclerosis complex (TSC) results from loss of a tumor suppressor gene - TSC 1 or TSC 2, encoding hamartin and tuberin, respectively. These proteins formed a complex to inhibit mTORC1-mediated cell growth and proliferation. Loss of either protein leads to overgrowth lesions in many vital organs.

2015年10月22日 · In this study, we generated a novel mutant mouse line (NEX- Tsc2) in which the Tsc2 gene is deleted specifically in postmitotic excitatory neurons of the developing forebrain. Homozygous mutant mice failed to thrive and died prematurely, whereas heterozygous mice appeared normal.

2022年12月1日 · In this study, we generated a novel mouse model involving conditional deletion of Tsc1 in lysozyme 2 (Lyz2)-positive cells which replicated several features of brain lesions including epileptic seizures, megalencephaly, highly …

2004年10月13日 · Approximately 80% of the Tsc2 −/− MEFs expressing exogenous tuberin and approximately 60% of the Tsc1 −/− MEFs expressing exogenous hamartin had no detectable phosphorylated S6 (pS6 ...

2011年11月4日 · Mutant SVZ showed significant reduction in both Tsc1 and Tsc2 proteins, with no activation in pS6 or difference in pERK or pAkt (Figure 5E). Whereas accumulation of Type A neuroblasts could be detected dorsally, alterations in Type B SC distribution were observed along the entire wall of the SVZ ( Figure 5 F).

2019年12月24日 · In this study we demonstrated that rapamycin, at a clinically tolerable concentration (10 nM), inhibited the phosphorylation of S6, but not the critical eIF4E releasing Thr 37/46 phosphorylation sites of 4E-BP1 in TSC2-deficient LAM-derived cells. We also characterized the abundant protein expression of peIF4E within LAM lesions.

(A) pS6 expression in Tsc2 hypomorphic mice (KC+) is three times higher than in CC− littermate controls, indicating a strong mTORC1 upregulation. (B) KC+ mice treated with TC1 (3 mg/kg) show a significant reduction of pS6 levels at 3‐h postadministration.

2023年8月6日 · Levels of phosphorylated S6 (pS6) and phosphorylated AKT (pAKT) detected via Western blot in whole brain lysates were compared across Tsc2 hypomorphic genotypes (CC− vs. KC+) and after treatment with either RAD001, TC1, or vehicle as a readout of the mTORC1 and mTORC2 complex activity, respectively. pS6 and pAKT levels, normalized to total ...

Surgically resected human cortical tubers and nondysplastic epileptic cortical samples were analyzed by double-label immunocytochemistry for coexpression of neuronal and glial markers, the TSC1/TSC2 pathway downstream molecule phospho-S6 (pS6) and GluR subunits, and compared with control cortical tissue.