2016年9月25日 · BCIP/NBT碱性磷酸酯酶显色试剂盒(BCIP/NBT Alkaline Phosphatase Color Development Kit)是一种用于免疫组化显 色、Western等膜显色和诱导多功能干细胞iPS鉴定等的试剂盒。

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product. BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

Use BCIP as precipitating substrate for alkaline phosphatase.

显色试剂盒(BCIP/NBT Color Development Kit)是一种用于免疫组化显色、Western等膜显色和诱导多功能干细胞iPS鉴定等的试剂盒。 即5-溴-4-氯-3-吲哚基-磷酸盐（5-Bromo-4-Chloro-3-Indolyl Phosphate），在碱性磷酸酶（Alkaline phosphatase,AP）的催化作用下，BCIP会被水解产生强反应性的产物，该产物会和 NBT氯化硝基四氮唑兰（Nitroblue tetrazolium chloride）发生反应，形成不溶性的深蓝色至蓝紫色的沉淀NBT-formazan。 的鉴定，Western等结合有碱性磷酸酯酶的 …

Sensitive detection of alkaline phosphatase in blotting protocols, such as Southern, northern, and western blotting, and colony and plaque lifts. Immunohistochemistry and immunocytochemistry. Detection of nucleic acids, proteins, and glycoconjugates. NBT/BCIP may be used with both nitrocellulose and nylon membranes*. 2. How to Use this Product. 2.

用于碱性磷酸酶标记分子的比色检测，可使用5-溴-4-氯-3-吲哚基磷酸酯（bcip）和硝基蓝四唑（nbt）。 BCIP/NBT底物系统具有多种功能并用于多种应用，包括Northern Southern和Western blotting、 原位 杂交和免疫组化。

phosphatase conjugates.1-3 SIGMAFAST™ BCIP®/NBT tablets are useful in immunochemistry to detect alkaline phosphatase. Common uses are in Western blotting or dot blotting, and to a lesser extent in immunohistology. SIGMAFAST™ BCIP®/NBT tablets require no additional buffers or steps to prepare an active substrate solution.

Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

2024年6月2日 · 捷倍斯生物生产的 BCIP/NBT碱性磷酸酶显色试剂盒(BCIP/NBT Alkaline Phosphatase Color Development Kit-GBCBIO-E1161) 可用于免疫组化显色、Western等膜显色等的试剂盒。BCIP/NBT是碱性磷酸酯酶的常用底物。

BCIP (1) is the alkaline phosphatase (AP) substrate, which after dephosphorylation is oxidized by NBT to yield a dark-blue indigo pre-cipitating dye. NBT (2) is thereby reduced to a dark-blue precipitating dye and serves to intensify the color reaction making the detection. more sensitive.