2020年12月15日 · The atomic force microscopy (AFM) imaging of Tau fibrils was performed in ambient conditions at room temperature (25°C) using a JPK Nanowizard Ultraspeed atomic force microscope from JPK Instruments (Berlin, Germany) in amplitude modulation AFM (AM-AFM) with low force settings (80–90% of the free amplitude A ∼10 nm).

The possibility to analyze native samples without fixation, staining and in physiological buffer conditions, combined with the sub-nanometer resolution, makes AFM a versatile tool for the analysis of protein aggregation and amyloid structures. Here, we describe the application of AFM to study fibrillar Tau protein aggregates.

The atomic force microscopy (AFM) imaging of Tau fibrils was performed in ambient conditions at room temperature (25°C) using a JPK Nanowizard Ultraspeed atomic force microscope from JPK Instruments (Berlin, Germany) in amplitude modulation AFM (AM-AFM) with low force settings (80–90% of the free amplitude A ∼10 nm).

2012年12月26日 · We tackled the challenge of visualizing the fuzzy coat of Tau fibrils by applying immunogold-TEM and high-resolution AFM in the force-volume mode (FV-AFM), which is particularly suited to imaging single biomolecules at much improved force sensitivity and to mapping their physicochemical properties at nanometer resolution (≈1–2 nm ...

2024年12月31日 · Atomic force microscopy (AFM) of Tau after 72 h of fibrillization revealed the formation of different aggregated structures, including long and short fibrils as well as oligomers (Fig. 1 C).

2018年11月1日 · Atomic force microscopy (AFM)-based approaches provide multifunctional nanotools to measure a wide variety of mechanical properties of living systems and to apply to...

Microtubule-associated protein tau (MAPT) is a constituent of axonal cytoskeletons in normally functioning neurons, while self-assembly of microtubule-unbound tau into fibrils is a...

2024年2月8日 · This project evaluates the physical properties of the projection domains of naturally occuring tau isoforms with different numbers of N terminal inserts using atomic force microscopy (AFM) in order to learn more about the impact of the inserts.

2023年8月6日 · α 突触核蛋白原子力显微镜（AFM）实验操作步骤（适用于 tau, Aβ 等其他活性蛋白）

2024年2月21日 · This review highlights the use of AFM as a suitable research technique for the study of cellular damages in tauopathies, even in early stages, allowing elucidation of pathogenic mechanisms of these diseases.