In molecular biology, TBST (or TTBS) is a mixture of tris-buffered saline (TBS) (a buffer solution) and Polysorbate 20 (a polysorbate-type nonionic surfactant). Polysorbate 20 is also known as …

TBST for Western Blotting Tris-buffered saline with 0.1% Tween ® 20 detergent (TBST) is an effective wash buffer for many immunoassays. To make 1 L of TBST wash buffer, add 100 mL …

Add BSA to Tris-buffered saline (TBS) or TBST (TBS containing 0.1% Tween 20). Mix well and filter. Failure to filter can lead to spotting, with tiny dark dots contaminating the blot during …

TBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Tris Buffered Saline with Tween® 20 is an optimal formulation of …

RIPA buffer contains the ionic detergent sodium deoxycholate as an active constituent and is particularly useful for nuclear membrane disruption for nuclear extracts. A RIPA buffer gives …

Prepare a working solution of TBS (1×, pH 7.4) by diluting the 20× stock in Milli-Q water. For TBST, add 1 mL of Tween 20 per liter of TBS. Stir constantly until homogenous. What's this?

Place the cell culture dish in ice and wash the cells with ice-cold Tris-buffered saline (TBS). Aspirate the TBS, then add ice-cold RIPA buffer (1 ml per 100 mm dish). Scrape adherent …