Electrophoretic mobility shift assays (EMSA) of the Arabidopsis TCP4 protein unveil that a 58-residue-long TCP domain is essential and sufficient for interacting with DNA and possesses DNA-binding parameters comparable to those of canonical bHLH proteins. 109 Furthermore, the residues that are crucial for DNA binding and homodimerization are ...

2019年10月1日 · By combining loss and gain-of-function analysis of TCP proteins with biochemical experiments, we here show that TCP4 suppresses trichome initiation in Arabidopsis leaves by directly activating the transcription of TCL1 and TCL2.

2023年9月13日 · Gel electrophoresis mobility shift assay (EMSA) results indicated that TCP4 bound to the promoter of CRC or SPT (Fig. 4h), suggesting that TCP4 directly...

2022年7月11日 · We further confirmed that TCP4 bound directly to cis elements by an electrophoretic mobility shift assay (EMSA) (Figure 5 Q). These results clearly demonstrate that TCP4 promotes the conversion of chloroplasts into leucoplasts by repressing chlorophyll biosynthesis genes directly or indirectly through its influence on other TFs, such as SOC1.

2017年11月22日 · Here, we show that the miR319-regulated class II TCP proteins, notably TCP4, suppress trichome branching in Arabidopsis leaves and inflorescence stem by direct transcriptional activation of GLABROUS INFLORESCENCE STEMS (GIS), a known negative regulator of trichome branching.

2010年4月2日 · We report biochemical characterization and DNA binding properties of a TCP member in Arabidopsis thaliana, TCP4. We have shown that the 58-residue domain of TCP4 is essential and sufficient for binding to DNA and possesses DNA binding parameters comparable to canonical bHLH proteins.

2020年7月6日 · An electrophoretic mobility shift assay (EMSA) using the GhFSN1 and GhCESA7 promoter fragments showed that GhTCP4A indeed bound to the promoters of these genes (Figure 6 F and Supplemental Figure 16), and the binding reaction was an endothermic process, as determined by an isothermal titration calorimetry (ITC) assay (Supplemental Figure 16 C).

2016年1月1日 · Electrophoretic mobility shift assay (EMSA) of AtTCP4 in the presence of chemicals that discriminate between the major and minor grooves of the DNA double-helix further suggested that, like canonical bHLH proteins, the basic region binds the DNA major groove (Aggarwal et al., 2010).

To demonstrate direct dimer formation by DNA-bound TCP4, we performed EMSA experiments using radioactively labeled target DNA and two forms of TCP4 protein with different molecular masses. When used individually, purified TCP4Δ3 and TCP4Δ3-MBP retarded the mobility of the target DNA to different extents.

2024年3月20日 · ‐LIPOXYGENASE2 (UVR8 ‐TCP4‐LOX2) module regulates UV‐B tolerance dependent on JA signaling pathway in Arabidopsis thaliana. We show that the nucleus‐localized UVR8 physically interacts with TCP4 to increase the DNA‐binding activity of TCP4 and upregulate the JA biosyn-thesis gene LOX2.