TEM-1 β-lactamase is one of the most well-known antibiotic resistance determinants around. It confers resistance to penicillins and early cephalosporins and has shown an astonishing functional plasticity in response to the introduction of novel drugs derived from these antibiotics.

TEM-1 β-lactamase is a class A enzyme encoded by the bla TEM-1 gene, which is present on the Tn3 transposon [8, 9]. Epidemiological studies have shown TEM-1 to be the most common plasmid-mediated β-lactamase in Gram-negative bacteria [3].

2010年11月1日 · TEM-1 β-lactamase is one of the most well-known antibiotic resistance determinants around. It confers resistance to penicillins and early cephalosporins and has shown an astonishing functional plasticity in response to the introduction of novel drugs derived from these antibiotics.

TEM-1是革兰阴性菌中最常见的一种β-内酰胺酶，在氨苄西林耐药的大肠埃希菌中，90%的菌株是由于产生了TEM-1。TEM-2 与TEM-1仅一个氨基酸不同（39位氨基酸TEM-1：Gln→TEM-2：Lys），产TEM-2的菌株明显少于TEM-1菌株（占5%）。TEM-1、TEM-2可以水解青霉素类和第一代头孢菌素 ...

TEM-1 β-lactamase is a class A enzyme and is the most common plasmid-encoded β-lactamase in Gram-negative bacteria (8). TEM-1 β-lactamase efficiently hydrolyzes penicillins and many cephalosporins, but it is not an effective catalyst for extended spectrum cephalosporin turnover (9).

2013年7月22日 · Using TEM-1 as a model enzyme, we were able to uncover some universal determinants of mutation effects, to quantify how powerful they were to explain the impact of mutations and to define a simple model that could capture …

2005年4月20日 · One of the best-studied examples of a class A beta-lactamase is Escherichia coli TEM-1 beta-lactamase. In this class of enzymes, the active-site serine residue takes on the role of a nucleophile and carries out beta-lactam hydrolysis. Here, the structures of the wild-type and the S70G enzyme determined to 1.55 and 2.1 A, respectively, are ...

2022年1月1日 · Through analysis of the mutational data and structures, we propose two evolution routes for the TEM-1 variants. The first is the G 238 S route (route 1), in which a single amino acid substitution of

2018年10月20日 · Escherichia coli β-lactamase TEM-1 is potentially useful in the antibody-directed enzyme/prodrug therapy (ADEPT), converting nontoxic prodrugs to toxic agents. The produced toxin would kill cancer cells, when the enzyme is attached to a …

2010年4月24日 · TEM-1β-内酰胺酶是周围最著名的抗生素耐药性决定因素之一。 它赋予了对青霉素和早期头孢菌素的抗性，并且由于引入了源自这些抗生素的新药而显示出惊人的功能可塑性。 自1960年代发现以来，全世界医院和诊所已经分离出170多种TEM-1变体，这些变体具有不同的氨基酸序列，通常具有抗性表型。 除了这种有据可查的“自然”进化，TEM-1的体外进化已成为许多实验研究的焦点。 在这篇综述中，我们比较了TEM-1的自然进化和实验室进化，以解决这个问 …