2012年4月1日 · GFP as fusion tag for protein localization. GFP can be used as a tag when it is fused to the protein of interest (protein x), allowing its detection in the cytoplasm, in specific organelles or at the plasmatic membrane. It can be detected by TEM after immunogold staining.

2020年4月29日 · Incorporating an additional non-pH-sensitive red fluorescent tag allows visualization of the exosome lifecycle, including multivesicular body (MVB) trafficking, MVB fusion, exosome uptake and...

The Aequorea victoria green fluorescent protein (GFP) and fluorescent derivatives are popular tags to label chosen proteins through genetically made fusion proteins and to localize them with FM even in living cells and organisms.

After pegylation, the modified AuNPs are able to non-covalently anchor Nb dimers and the subsequent complexes demonstrate the ability to form immunogold label GFP-protein fusions within various subcellular locations. These tools open an avenue for precise localization of targets at high resolution by electron microscopy.

2021年8月11日 · We have shown the feasibility and efficiency of this method to detect various proteins in Drosophila ovarian follicles such as nuclear proteins, proteins associated with endocytic vesicles, plasma membranes or nuclear envelopes.

2021年9月24日 · Targeting Signal tagged GFP (MTS-GFP), GFP tagged-PCNA - Interacting Pep tide (GFP-PIP), and H2B-GFP are imaged. This is on the one hand definitely showing things that haven't been seen...

2021年9月24日 · HeLa cells were transiently transfected with DNA plasmids to express different GFP-protein fusions indicated as follows: β-galactosidase-GFP (β-Gal-GFP), mitochondria-targeting signal tagged GFP (MTS-GFP), and GFP tagged-PCNA-interacting peptide (GFP-PIP).

We first present a protocol to synthesize 5-nm-diameter gold nanoparticles and functionalize them with a nanobody, a single-domain antibody from camelid, targeting the widespread green fluorescent protein (GFP)-tagged proteins with a high affinity.

Although green fluorescent protein has been similarly programmed in as a "tail" fusion protein for following proteins in a cell by light microscopy, EM requires a dense label, such as gold. One may use anti-GFP gold labels, but another approach is to derivatize gold nanoparticles with the binding couple for the tags commonly used in protein ...

Methods: By mutagenesis, a smaller size tetracysteine (TC) tag (C-C-P-G-C-C) that could be bound with a biarsenical fluorescent dye was genetically inserted at different cell epitopes of HBV core protein expressed in transfected cells.