The Tet-Off system allows silencing of gene expression by administration of tetracycline (Tc) or tetracycline-derivatives like doxycycline (dox), whereas the Tet-On system allows activation of gene expression by dox.

Tet-One inducible expression systems are the only commercially available single-vector systems that can deliver 3–4 orders of magnitude difference in expression levels between induced and uninduced states. These systems are available in lentiviral-, retroviral-, …

Apr 1, 2015 · In both the Tet-Off and Tet-On systems, expression of the transcriptional activator can be regulated both reversibly and quantitatively by exposing the transgenic animals to varying concentrations of tetracycline (Tc), or Tc derivatives such as doxycycline (Dox).

We offer several plasmid and viral delivery options for the Tet-On 3G system, plus promoters that allow for consistent long-term expression of the Tet transactivator.

The Tet-On 3G Systems are inducible gene expression systems for mammalian cells. Target cells that express the Tet-On 3G transactivator protein and contain a gene of interest (GOI) under the control of a TRE3G promoter (PTRE3G) will express high levels of your GOI, but only when cultured in the presence of doxycycline (Dox) (Figure 1). Figure 1.

Jan 14, 2020 · With the “Tet-Off” and “Tet-On” systems, the expression of a gene of interest, or even a short hairpin RNA, can be controlled by the presence (or absence) of tetracycline or one of its derivatives (such as doxycycline).

The Tet-Off system allows silencing of gene expression by administration of tetracycline (Tc) or tetracycline-derivatives like doxycycline (dox), whereas the Tet-On system allows activation of gene expression by dox.

Tetracycline (Tet) technology allows precise, reversible, and efficient spatiotemporal control of gene expression. This “on demand” gene induction mimics disease onset and disease progression. When coupled with Cre recombinase, Tet technology allows one to selectively shut down target gene expression.

Oct 9, 2018 · The Tet-OFF system activates downstream gene expression in the absence of Dox, whereas the Tet-ON system activates it in the presence of Dox. The original Tet system has been improved significantly for use in eukaryotic cells, including mammalian cells.

Our study echoes ongoing challenges faced by fundamental researchers working with β-cells and highlights the need for consistent and comprehensive controls when using the tetracycline-controlled transactivator systems (Tet-On or Tet-Off) for genome editing.