TEV Protease is a highly specific cysteine protease. The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ S; however, the amino acid in the P1’ position can also be G, A, M, C, or H (1).

tev是一种用来切除融合蛋白上亲和标签的常用工具酶，具有很强的位点特异性，严格识别七氨基酸序列exxyxq↓(g/s)，切割位点在谷氨酰胺和甘氨酸/丝氨酸之间。

TEV Protease(His-tag)是一种在大肠杆菌中重组表达的带His标签(6X His tag)的烟草蚀纹病毒(Tobacco Etch Virus, TEV)的半胱氨酸蛋白酶，能特异性地识别七肽序列Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser，并在Gln和Gly/Ser氨基酸残基之间进行酶切，常用于去除融合蛋白的Glutathione S-transferase (GST ...

TEV protease (EC 3.4.22.44, Tobacco Etch Virus nuclear-inclusion-a endopeptidase) is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV). [1] It is a member of the PA clan of chymotrypsin-like proteases. [2]

TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. It is

TEV Protease 是一种广泛应用于切除重组蛋白融合标签的蛋白酶，以其高度的位点特异性而著称。 它严格识别七氨基酸序列EXXYXQ↓ (G/S)，在谷氨酰胺和甘氨酸或丝氨酸之间进行精确剪切，其中最常见的七氨基酸序列为：Glu-Asn-Leu-Tyr-Phe-Gln↓-Gly。 这种特异性确保了蛋白加工过程中的精确性和效率。 图 2.TEV蛋白酶作用原理图 [2] 翌圣 UCF.ME ® rTEV Protease 是一款经过基因工程改良和精细纯化的重组蛋白酶，它不仅保留了天然TEV酶的完整功能活性，而且在更 …

2018年7月30日 · Here we characterize a minimal, modular platform for engineering membraneless IDP-based compartments, enzymatically trigger liquid mixing and demixing, and demonstrate programmable cargo...

Histidine-tagged recombinant, expressed in E. coli, rigorously purified via three rounds of chromatography. TEV protease, originally isolated from Tobacco Etch Virus, is a member of the family of C4 peptidases. Three proteolytic enzymes are released from the N-terminus of the TEV polyprotein: P1, HC-Pro, and Nuclear Inclusion a (NIa).

tevprotease（tev酶）是来源于烟草蚀纹病毒(tev)的重组蛋白酶，经改造后与天然tev酶相比其稳定性更好，常被用来切除纯化 后融合蛋白的亲和标签。tev酶具有很强的位点特异性，能够识别exxyxq(g/s)的七氨基酸序列，最常用的是enlyfqg，其切割位点

2008年7月9日 · The TEV protease cuts between Gln and Gly. It is often used in Ni-Nta protein column purifications to cut off the binding tag. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.