Temperature gradient gel electrophoresis (TGGE) and denaturing gradient gel electrophoresis (DGGE) are forms of electrophoresis which use either a temperature or chemical gradient to denature the sample as it moves across an acrylamide gel. TGGE and DGGE can be applied to nucleic acids such as DNA and RNA, and (less commonly) proteins.

TGGE是一种有效的分离DNA、RNA或者蛋白的手段。 它利用了不同分子在温度改变下构象的差别进行分离。 另外一种类似的技术是 变性梯度凝胶电泳 (denaturing gradient gel electrophoresis, DGGE)，则是利用不同分子在不同浓度的变性剂的作用下失活实现的。

溫度梯度膠體電泳 (t emperature g radient g el e lectrophoresis, TGGE)是 電泳 技術的一種，通過物質在不同溫度下性質的區別進行分離。 TGGE是一種有效的分離 DNA 、 RNA 或者 蛋白 的手段。 它利用了不同分子在溫度改變下 構象 的差別進行分離。

Temperature Gradient Gel Electrophoresis (TGGE) is a form of electrophoresis in which temperature gradient is used to denature molecules as they move through either acrylamide or agarose gel. TGGE can be applied to analyze DNA, RNA, protein-DNA complexes, and, less commonly, proteins. Separation of …

Denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) are electrophoretic techniques used to separate PCR-amplified DNA fragments of similar lengths according to differences in melting temperatures.43 Since a melting temperature of a DNA fragment is determined by its G + C content, DGGE/TGGE are ...

2010年1月1日 · TGGE is based on detecting differences in the electrophoretic mobility between molecules that may differ only at a single position. DNA fragments produced by the polymerase chain reaction (PCR) are subjected to electrophoresis through a linearly increasing gradient of temperature (or concentration gradient of denaturing agents such as urea and ...

2011年1月7日 · TGGE is a technique used to separate DNA or proteins based on temperature gradients. It allows complex samples to be separated into distinct bands based on small sequence variations. The document discusses the methodology …

2018年4月4日 · TGGE is a method of electrophoresis used to separate proteins or nucleic acids (DNA and RNA) across a temperature gradient. TGGE is based on the principles that the structure of proteins and DNA changes with temperature and that these changes can be characterized by their movement trough a gel.

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This protocol describes a TGGE analysis with a perpendicular temperature gradient and should be used to optimize the parameters to obtain the maximum difference in mobility of different nucleic acids at the respective transition temperatures (see Notes 3 – 5 ).