A ready-to-use mix for SYBR Green qPCR, containing ROX reference and suitable for all qPCR instruments without adjusting the concentration of ROX. 1 vial of lyophilized qSTAR qPCR …

2024年8月20日 · According to the flow cytometric and quantitative reverse-transcription PCR (RT-qPCR) results, sTim-3 inhibited IFN-γ and TNF-α secretion upon anti-CD3/CD28 stimulation in …

2021年6月9日 · Here, using conditional knockouts of TIM-3 together with single-cell RNA sequencing, we demonstrate the singular importance of TIM-3 on dendritic cells (DCs), …

2024年2月13日 · Here, we discuss the distinct functions of LAG-3, TIM-3, and TIGIT, including their contributions to the regulation of immune cells beyond T cells, their roles in disease, and …

2022年11月18日 · In vivo limiting dilution assay further confirmed the enhanced self-renewal capabilities of SB glioma cells after Tim3 overexpression (Figures 2 N and 2O). qPCR assay in …

Currently, experimental medicine involving TIM-3 has significantly enhanced the anti-tumor effect and improved patient survival. In this work, we summarized clinical trials incorporating TIM-3 …

TIM-3 is upregulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg). Its binding to Galectin-9 …

1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions). Verified forward and reverse primers for analyzing the quantitative expression of …

2.Stringent screening and validation process: Sensitivity, amplification efficiency and specificity of qPCR primers are screened using plasmid standards, and confirmed with positive tissues or …

Immunohistochemical analysis, quantitative real-time polymerase chain reaction (RT-qPCR) and western blot were performed for the detection of Tim-3 in different tissues. The …