Tetramethylphenylenediamine (TMPD) is an organic compound with the formula C 6 H 4 (N(CH 3) 2) 2. It is most studied of three isomers of this formula. It is a colorless solid. ... The hydrochloride salt of TMPD has been used as a redox indicator in the oxidase test and is also used in electron transport chain analysis as it is capable of ...

(a) Tetra-methyl-p-phenylenediamine dihydrochloride (TMPD), the oxidase reagent, is electron rich (reduced) and has no color. (b) In bacteria that contain the enzyme cytochrome oxidase, one electron from each of four cytochrome c molecules will be temporarily transferred to the enzyme.

The oxidase test is used to determine whether an organism possesses the cytochrome c oxidase enzyme. The test is used as an aid for the differentiation of Neisseria, Moraxella, Campylobacter and Pasteurella species (oxidase positive). It is also used to differentiate pseudomonads from related species. [1]

The colorimetric identification of pathogenic and non-pathogenic bacteria in cell culture is commonly performed using the redox mediator N,N,N′,N′-tetramethyl-para-phenylene-diamine (TMPD) in the so-called oxidase test, which indicates the presence of bacterial cytochrome c oxidases. The presented study demonstrates the ability of ...

提出的研究证明了电化学利用TMPD检测细菌并量化细菌细胞色素c氧化酶活性的能力。 对模型生物枯草芽孢杆菌进行循环伏安法研究和计时电流法测量得出针对单个细菌计算的周转数。 此外，在需氧培养的大肠杆菌中发现了痕量的细胞色素C氧化酶，据我们所知，目前尚无其他技术能够在分子生物学中进行定量。 报道的技术可以应用于多种致病细菌，并有可能在未来的生物传感技术中使 …

2021年3月23日 · Oxidase test uses disks impregnated with a reagent such as N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD) or N,N-dimethyl-p-phenylenediamine (DMPD), which is also a redox indicator. The reagent is a dark-blue to …

TMPD (N,N,N′,N′-tetramethyl-para-phenylene-diamine) is used in cell culture and microbiology to differentiate organisms that exhibit cytochrome c oxidase activity and to distinguish between Gram-negative and Gram-positive pathogenic and non-pathogenic bacteria.

1978年12月1日 · TMPD (E= +0.26 V at pH 7.0) acts as an artificial electron donor to the terminal portion of aerobic respiratory systems [4], and the ability to oxidise TMPD, reduced by ascorbate, has been widely used as a quantitative assay of terminal oxidase activity [5].

2024年5月9日 · The oxidase test uses a colorless reagent tetra-methyl- p-phenylenediamine dihydrochloride (TMPD), which acts as an artificial donor for cytochrome c. When cytochrome c oxidase is present, it oxidizes TMPD, changing it from colorless to …

Tetramethylphenylenediamine (TMPD) is an organic compound with the formula C 6 H 4 (N(CH 3) 2) 2. It is most studied of three isomers of this formula. It is a colorless solid. ... The hydrochloride salt of TMPD finds use as a redox indicator in the oxidase test and is also used in electron transport chain analysis as it is capable of donating ...