2001年7月1日 · These vectors insert Tn 7 into a specific, neutral intergenic region immediately downstream of the gene encoding glucosamine-6-phosphate synthetase (GlmS) in the tested …

2001年11月1日 · When the TnsD protein is utilized with the core machinery, insertions are directed into a single site in the bacterial chromosome, called att Tn7. This site is highly …

2005年5月20日 · Here we describe a series of small, fully sequenced mini-Tn7 vectors, which contain selection markers that, after transposition, can be excised from the bacterial …

2013年6月6日 · Construction of mini-Tn 7-lux reporter vectors with oriT for mobilization and a trimethoprim resistance selection marker. Mini-Tn 7 vectors have been shown to be useful in …

2006年6月27日 · Broad host-range mini-Tn 7 vectors facilitate integration of single-copy genes into bacterial chromosomes at a neutral, naturally evolved site. Here we present a protocol for …

These vectors insert Tn7 into a specific, neutral intergenic region immediately downstream of the gene encoding glucosamine-6-phosphate synthetase (GlmS) in the tested fluorescent …

These vectors insert Tn7 into a specific, neutral intergenic region immediately downstream of the gene encoding glucosamine-6-phosphate synthetase Ž GlmS . in the tested fluorescent …

2001年8月1日 · These vectors insert Tn7 into a specific, neutral intergenic region immediately downstream of the gene encoding glucosamine-6-phosphate synthetase (GlmS) in the tested …

In this study, mini-Tn 7 vectors with zeocin and apramycin selection markers were created by cloning the ble and aac (3)-IV genes, respectively, enabling either inducible gene expression …

2015年3月25日 · In this work, we have established a standardized heterologous expression device in which the promoter strength is the only variable; the remaining parameters of the …