Genomic analysis of a Trichoplusia ni Betabaculovirus (TnGV
The complete genome of a Trichoplusia ni granulovirus (TnGV) is described and analyzed. The genome contains 175,360 bp (KU752557), becoming the third largest genome within the genus Betabaculovirus, smaller only than the Xestia c-nigrum GV (XecnGV) (178,733 pb) and the Pseudaletia unipuncta GV (Psun …
Enhancement of Bacillus thuringiensis Toxicity to Lepidopterous …
Feb 1, 2001 · The enhancin from the Trichoplusia ni (Hübner) granulovirus (TnGV) is a 104-kDa viral protein that can significantly increase the virulence of several baculoviruses. Enhancin can destroy the structural integrity of the peritrophic membrane (PM) of lepidopterous larvae, resulting in more efficient penetration of the PM by virions and avoidance ...
Genomic analysis of a Trichoplusia ni Betabaculovirus (TnGV
Aug 30, 2017 · The complete genome of a Trichoplusia ni granulovirus (TnGV) is described and analyzed. The genome contains 175,360 bp (KU752557), becoming the third largest genome within the genus Betabaculovirus, smaller only than the Xestia c-nigrum GV (XecnGV) (178,733 pb) and the Pseudaletia unipuncta GV (PsunGV) (176,677 pb) genomes.
Aug 1, 2021 · Trichoplusia ni granulovirus (TnGV) genome in combination with the PIG bombardment technique to obtain a recombinant virus. A repair vector was developed which, when evaluated, showed the potential of baculoviruses to produce recombinant proteins when they do not have insect cell lines for genetic modification. Materials and methods
Physical mapping and identification of interspersed homologous ...
By restriction endonuclease and Southern blot analysis of cloned or genomic viral DNA fragments, a complete physical map of TnGV was constructed for BamHI, EcoRI, PstI and XhoI. Three interspersed homologous regions (ihs1-ihs3) were identified …
Genomic analysis of a Trichoplusia ni Betabaculovirus (TnGV) with …
Dec 1, 2017 · In the present study, we constructed a recombinant Autographa californica NPV (AcMNPV) that expressed the Trichoplusia ni GV (TnGV) enhancin 3 (En3), with the aim of increasing the presence of ...
Molecular characterization and expression of the Trichoplusia ni ...
A putative DNA helicase gene from the granulovirus of Trichoplusia ni (TnGV) was cloned, sequenced, and compared with the corresponding gene of several multinucleocapsid nucleopolyhedroviruses (MNPVs) including those from Autographa californica (AcMNPV), Orgyia pseudotsugata (OpMNPV), Bombyx mori (B …
A recombinant baculovirus TnGV in Trichoplusia ni larvae ... - bioRxiv
Aug 2, 2021 · In this work, the CRISPR/Cas9 system was used to edit the genome of Trichopusia ni granulovirus (TnGV) and transformation of insects by the PIG bombardment method. A homologous repair vector (pTnGV101) was designed with regions orf5 and orf7, as well as sgRNA flanking TnGV P10 of this virus.
Replication of the Trichoplusia ni granulosis and nuclear polyhedrosis ...
Jul 30, 1986 · Newly established Trichoplusia ni (cabbage looper) embryonic cell lines were infected with T. ni granulosis virus (TnGV) and T. ni nuclear polyhedrosis virus (TnSNPV). Infection of cultured cells with TnGV was ascertained by peroxidase-antiperoxidase staining, DNA slot-blot hybridization, and transmission electron microscopy.
A baculovirus anti-apoptosis gene homolog of the Trichoplusia …
An inhibitor of apoptosis (iap) gene homolog (Tn-iap) of the Trichoplusia ni granulovirus (TnGV) was cloned, sequenced and mapped on the genome of TnGV. Tn-iap encoded a protein (Tn-IAP) of 301 amino acids with a predicted molecular mass of 35 kDa.