Genomewide cDNA clone collection, covering Human, Mouse and Rat. 90% of gene loci are in stock. Every gene is offered in two different tags, Myc-DDK tag (DDK is the same as Flag tag) and GFP tag, facilitating gene expression detection and subcellular localization.

TrueORF Gold is OriGene's collection of premium cDNA clones that have passed the ultimate tests: expression validation & sequence verification. Using non-validated clones can result in inaccurate or absent expression. Don't waste your time and valuable resources. Order a TrueORF Gold clone and start your experiment today!

One common use of open reading frames (ORFs) is as one piece of evidence to assist in gene prediction. Long ORFs are often used, along with other evidence, to initially identify candidate protein-coding regions or functional RNA -coding regions in a DNA sequence. [5] .

2020年8月14日 · ORF克隆：一个全长ORF（开放阅读框）克隆，是指一个插入有编码全长蛋白质的DNA 片断的质粒。 该插入DNA 片断只含有编码一个全长蛋白质的序列（从起始密码到终止密码），而不包含有5′或3′端的非翻译区（UTRs）或内含子。 怎么？ 概念不好理解？ 没事，看下面的图：ORF就是基因的开放阅读框，没有5' 和3' UTR序列。 是不是感觉很简单呢？ cDNA 克隆最主要的用途就是大量复制，并保存单一cDNA信息，构建cDNA文库，亚克隆等。 也由于 …

TrueORF ® cDNA clones enable the expression of the encoded transcript as C-terminally tagged protein with Myc and FLAG ® epitopes. They facilitate multiple applications that utilize an anti-tag antibody, such as protein expression, protein purification and subcellular localization.

Flagship products include: - TrueORF Gold cDNA clones: fully sequenced and expression validated - UltraMAB monoclonal antibodies: the only antibodies tested for specificity - TrueMAB antibodies:...

2020年5月26日 · 单拷贝基因指在基因组中只出现一次，多是编码蛋白质的基因，真核生物中有25%~50%的基因是以单个基因存在的，而其余编码蛋白质的基因以基因家族形式存在；基因家族是来源于同一个祖先，由一个基因通过基因重复而产生两个或更多的拷贝而构成的一组基因，它们在结构和功能上具有明显的相似性，编码相似的蛋白质产物，欲更详细了解基因家族形成、特征请参见课程：充电课-限时免费领取《基因家族分析详解课程》。 5. 假基因也叫伪基因，他是基 …

To provide convenience to researchers, the replication-incompetent Lenti-ORF particles are available with different C-terminal tags (Myc-DDK and mGFP). Some particles also contain puro selection marker. The viral particles are filtered through 0.45μm filter and titrated via a HIV p24 antigen ELISA assay (OriGene cat# TR30038).

TrueClones® are full-length cDNA clones derived from human, mouse, and rat cDNA libraries. TrueClones® are ideal for over-expression of native proteins for functional studies. For over-expression of tagged proteins, OriGene offers TrueORF® with built-in tags for convenient detection and purification. » Search for your cDNA Clones (Browse All)

2018年6月1日 · ORF 克隆是指从样本组织或细胞中抽提总 RNA，反转录，设计合成特异性引物后，通过 RT-PCR 扩增获得目的基因的 DNA 序列，获得编码全长蛋白质序列的 DNA 序列：即从起始密码 ATG 到终止密码子，不含 5′或 3′UTRs 非翻译区）及内含子序列。 技术应用. 目的基因的表达研究. 基因、蛋白的功能研究. 实验流程. 实验结果展示. A B C. 图1 目的基因的ORF克隆实验结果. A:样本1和样本2的RNA抽提电泳结果 ；B 不同扩增条件下目的片段的PCR扩增结果；C 目的 …