2007年1月14日 · Here, Arabidopsis hypocotyl microtubules wershee labelled with AtEB1a–GFP (Arabidopsis microtubule end-binding protein 1a) or GFP–TUA6 (Arabidopsis α-tubulin 6) to record long cycles of...

2013年10月21日 · To test whether tubulins phosphorylated by PHS1 polymerize to microtubules in vivo, we coexpressed GFP-TUA6 (which labeled α-tubulin) and mCherry-TUB6 (which labeled β-tubulin) in transgenic Arabidopsis plants (Figures 5 A and 5B).

2005年6月22日 · We have previously reported that, when GFP was fused to the N-terminus of the Arabidopsis α -tubulin 6 isoform (TUA6) and expressed constitutively under the control of the cauliflower mosaic virus (CaMV) 35S promoter in transgenic Arabidopsis plants, the fusion protein was incorporated into and fluorescently labeled microtubules in aerial ...

2022年8月17日 · For live-confocal imaging of cortical microtubules, cotyledons stably expressing GFP-TUA6 (excitation at 488, emission 500–550 nm) and mCherry-TUA5 (excitation at 561 nm, emission 570–650 nm) were imaged using a Zeiss LSM880 confocal laser scanning microscope. ipga1-1 and ipga1-2 were crossed to the GFP-TUA6 line, generating ipga1-1 GFP ...

In quantitative terms, cortical microtubules of hypocotyl epidermal cells stably transformed with GFP-MBD or GFP-TUA6 exhibited alternating periods of growth succeeded by fast shrinkage (Fig....

2024年9月18日 · Encodes an alpha-tubulin isoform required for right handed helical growth. GFP-tagging of cell components reveals the dynamics of subcellular re-organization in response to infection of Arabidopsis by oomycete pathogens. Takemoto D, et al. Plant J, …

2024年5月20日 · To discard possible unspecific increases of GFP signal induced by the stretching, we used UBQ10::Venus-TUA6, which showed the same effect, and actin marker Fimbrin-YFP and 35 S::GFP (free cytosolic GFP), both showing no increase of fluorescence signal upon aspiration, respectively (Figures S5 A–S5G).

2023年10月24日 · While both MAP65-6 and MAP65-7 fusion proteins bound apparent microtubule bundles within the cortical arrays, not all GFP:TUA6 or mCherry:TUA6 microtubule structures were MAP65 labeled (Figures 1C, D, G, H).

Microtubules (MTs) were visualized in living cells of several tissues in transgenic Arabidopsis thaliana. The transformed Arabidopsis plant was obtained by infecting it with Agrobacterium tumefaciens carrying the GFP-TUA6 plasmid. The fluorescence of the MTs was due to the fluorescence of GFP-TUA6 that was polymerized into the MTs.

2021年5月11日 · Our findings reveal an organ-specific role of PIF proteins in regulating cytokinesis by BUB3.1 during apical hook development. To protect their newly formed fragile organs, dark-grown dicotyledonous plants form an apical hook when penetrating through the soil.