i) Lulus Penilaian Menengah Rendah /Sijil Rendah Pelajaran atau kelayakan yang diiktiraf setaraf dengannya oleh kerajaan. i) Tinggi sekurang-kurangnya 1.63m (lelaki) dan 1.57m (wanita) tanpa sepatu. ii) Berat badan sekurang-kurangnya 50kg (lelaki) dan wanita sekurang-kurangnya 48 kg.

The Ty1 retrotransposon of the yeast Saccharomyces cerevisiae integrates upstream of RNA polymerase III (Pol III)–transcribed genes, yet the primary determinant of target specificity has remained elusive.

2020年6月18日 · 在酿酒酵母基因组中发现有 5 种酵母长末端重复转座子(Transpon yeast，Ty)，分别命名为 Ty1-5 [2] ，其中 Ty1 [4] 在 Ty1 /Co-pia 家族中酵母基因组内含量最丰富，然而 Ty3/Gypsy 家族仅有 Ty3 一类转座子，Ty3 [5] 在编码的蛋白序列上与反转录病毒接近。

2016年3月18日 · Insertion of Ty1 elements into the S. cerevisiae genome, which occurs upstream of genes transcribed by RNA Pol III, requires the Ty1 element-encoded integrase (IN) protein. Here, we report that Ty1-IN interacts in vivo and in vitro with RNA Pol III-specific subunits to mediate insertion of Ty1 elements upstream of Pol III-transcribed genes.

我们通过界面突变体的生物物理分析以及体内 Ty1 转座和 p18m 限制来探索我们的发现。 我们的数据提供了对 Ty1 Gag 结构的洞察，并表明 p22/p18 如何通过组装阻塞机制在限制中发挥作用。

2023年3月28日 · The yeast Ty1 retrotransposon integrates upstream of genes transcribed by RNA polymerase III (Pol III). Specificity of integration is mediated by an interaction between the Ty1 integrase (IN1) and Pol III, currently uncharacterized at the atomic level. We report cryo-EM structures of Pol III in comp …

2012年9月21日 · Ty1 is transcribed by RNA pol II to form a 5.7-kb transcript, which functions as either gRNA for packaging into VLPs or mRNA for translation into Gag-p49 or Gag-Pol-p199. Gag accumulates in the cytoplasm to become the capsid protein of Ty1 VLPs. POL encodes the enzymes required for Ty1 transposition: PR, IN and RT.

2016年3月18日 · Insertion of Ty1 elements into the S. cerevisiae genome, which occurs upstream of genes transcribed by RNA Pol III, requires the Ty1 element-encoded integrase (IN) protein. Here, we report that Ty1-IN interacts in vivo and in vitro with RNA Pol III-specific subunits to mediate insertion of Ty1 elements upstream of Pol III-transcribed genes.

2012年12月17日 · Over the years, a number of reports have revealed that Ty1 integration occurs in a 1-kb window upstream of Pol III-transcribed genes with an approximate 80-bp periodicity between each integration hotspot and that this targeting requires active Pol III transcription at the site of integration.

Insertion of Ty1 elements into the S. cerevisiae genome, which occurs upstream of genes transcribed by RNA Pol III, requires the Ty1 element-encoded integrase (IN) protein. Here, we report that Ty1-IN interacts in vivo and in vitro with RNA Pol III-specific subunits to mediate insertion of Ty1 elements upstream of Pol III-transcribed genes.