Our results indicate that the process of adaptation by a Type II-A CRISPR–Cas system in Streptococcus thermophilus requires Cas1, Cas2, and Csn2. Surprisingly, we found that Cas9, …

2023年9月6日 · To reduce off-target risks, we perform a bioinformatic search and identify a high-fidelity Cas9 variant of the II-B subfamily from Parasutterella secunda (PsCas9). PsCas9 …

Plasmid pSpCas9 (BB)-2A-GFP (PX458) from Dr. Feng Zhang's lab contains the insert hSpCas9 and is published in Nat Protoc. 2013 Nov;8 (11):2281-308. doi: 10.1038/nprot.2013.143. Epub …

Our results indicate that the process of adaptation by a Type II-A CRISPR–Cas system in Streptococcus requires Cas1, Cas2, and Csn2. Surpris-thermophilus ingly, we found that …

Type II-A Cas9 has attracted significant attention since Streptococcus pyogenes Cas9 with the engineered single-guide RNA (sgRNA) has been developed as the revolutionary genome …

Chaperone-mediated autophagy (CMA) is a highly specific lysosomal-dependent protein degradation pathway. A critical molecular component of CMA is the lysosome-associated …

pME-Cas9-T2A-GFP Middle entry Gateway® vector encoding zebrafish codon-optimized S. pyogenes Cas9 with nuclear localization signals and with a C-terminal GFP. Sequence Author …

2024年7月12日 · In type II-A CRISPR systems, Cas9 uses the guide RNA to cleave target DNA sequences in the genome of infecting phages, and the tracrRNA to bind the promoter of cas …

2025年4月7日 · (B) Caco-2-ACE2 Cas9 cells from the panel 2A were infected with rSARS-CoV-2 Venus or Omicron BA.2 at an MOI of 0.1 or 1, fixed 24 h later and analysed by automated …

Our results indicate that the process of adaptation by a Type II-A CRISPR–Cas system in Streptococcus thermophilus requires Cas1, Cas2, and Csn2. Surprisingly, we found that Cas9, …