2025年3月3日 · Results: This study successfully achieved the combined detection of structural and functional connectivity in the V1 of mice. The rAAV1-hSyn-Cre virus was utilized for monosynaptic anterograde tracing.

2017年1月4日 · To test this, we first optogenetically activated the axon terminals of SC-sg neurons in LP (Figure 7D) in freely behaving mice, which resulted in similar, though somewhat weaker, expression of freezing (Figures 7E and 7F; V1-SC-LP), implicating LP’s role in driving SC-mediated freezing behavior.

分别在sc的上游视皮层、视网膜、听皮层及运动皮层注射aav1-cre，在sc注射aav1-cag-flex-gfp；可以看出被标记的不同上游支配的sc神经元所支配的下游也各有不同。

2020年4月20日 · Recombinant adeno-associated viruses (rAAVs) are efficient gene delivery vectors via intravenous delivery; however, natural serotypes display a finite set of...

2022年2月21日 · As a proof-of-principle, we aimed to demonstrate that postsynaptic neurons receiving both monosynaptic inputs can be selectively labelled in two distinct circuits: the retina/primary visual cortex...

2023年7月19日 · This study reveals the presence of a system of lateral HVAs that depends on the SC and that, in contrast to V1, responds preferentially to stimuli with positive visuo-motor divergence. The SC-dependency of each of these lateral HVAs matches the gradient of axonal arborizations arriving in visual cortex from SC through the pulvinar.

Here we describe the use of adeno-associated virus, serotype 1 (AAV1) as a tool for achieving robust, anterograde transsynaptic spread in a variety of unidirectional pathways.

2022年8月8日 · We injected the original virus (AAV1-hSyn-Cre) or the mutant virus (AAV1YF-hSyn-Cre) into the V1 region of Ai14 mice that express tdTomato under the presence of Cre protein. We then evaluated the effect of the YF mutations by counting the number of tdTomato labeled neurons in the SC.

Following two-step injection of AAV1-hSyn-Cre in either visual cortex (V1), retina, auditory cortex (A1), or primary motor cortex (M1) and AAV1-CAG-FLEX-GFP in SC, distinct patterns of axon labeling were observed throughout the brain.

2021年1月25日 · 目前常用的顺行跨单级突触示踪病毒主要有两种：HSV-1-H129- TK和AAV1。 HSV-1-H129- TK 是将HSV-1-H129复制所需的TK基因缺失掉，当感染神经元后，HSV-H129- TK能够正常表达荧光蛋白，但因缺乏TK蛋白而无法自我复制，不能传递到下一级神经元。 当利用腺相关病毒载体在细胞内表达TK蛋白后，病毒获得自我复制能力，通过突触结构传递到下一级神经元，由于下一级神经元中没有TK蛋白，因此病毒将不再继续传递下去，因而实现了顺行跨单级 …