To examine the relationship between V2R and AQP2 internalization more directly, we developed a cell line that expresses both V2R—GFP and c-myc-tagged AQP2. In these cells, AQP2 can be seen after fixation and immunostaining, while the V2R—GFP is directly fluorescent and is detectable without the need for immunostaining.

2021年7月1日 · Targeting the collecting duct water channel aquaporin 2 (AQP2) to the plasma membrane is essential for the maintenance of mammalian water homeostasis. The vasopressin V2 receptor (V2R), which is...

2025年1月1日 · AVP activates V2R, stimulating the formation of cyclic adenosine monophosphate (cAMP) and enhances the production of intratubular Ang II via distal tubule renin through V2R [7]. Ang II, in turn, can increase AVP-dependent cAMP production via protein kinase C activation [ 8 ].

Aquaporin-2 (AQP-2) has been shown to be a vasopressin-sensitive water channel in collecting duct (CD) cells of the kidney. To prove the role of the vasopressin V2 receptor (V2R) in the regulation of intracellular AQP-2 shuttling, we examined the acute effects of vasopressin and V2R antagonist on th …

2022年4月1日 · Triggers can be hormonal (e.g. vasopressin-induced localisation of AQP2 to the apical membrane of kidney collecting duct cells, V2R PDB ID: 7KH0) or environmental (e.g. hypoxia-induced localisation of AQP4 to the astrocyte plasma membrane). Internalisation through clathrin-dependent and clathrin-independent (such as caveolae) pathways reduce ...

近年来许多研究表明，梅尼埃病患者血浆avp水平超常地增高，avp可能通过v2r-camp-aqp2信号系统，使内耳aqp2表达升高，降低内淋巴囊上皮对水的重吸收，最终导致膜迷路积水，从而诱发眩晕、恶心、呕吐等类似运动病的症状 [5,6] 。

In the present study, we further investigated AQP2 accumulation at the cell surface, and compared it with V2R (VP type 2 receptor) trafficking using cells that express epitope-tagged AQP2 and V2R. Results: Endocytosis of V2R and of AQP2 are independent events that can be separated temporally and spatially.

2012年10月1日 · Vasopressin binds to its type 2 receptor (V2R) that is located in the basolateral membrane of collecting duct principal cells, and binding triggers the trafficking of AQP2 between the apical membrane and intracellular vesicles causing an increase of AQP2 at the apical membrane that allows high water permeability of the membrane.

2014年5月1日 · The arginine vasopressin (AVP) type 2 receptor (V2R) is unique among AVP receptor subtypes in signaling through cAMP. Its key function is in the kidneys, facilitating the urine concentrating mechan...

There are over 40 AQP2 mutations [236,237] known and described to cause phenotypic NDI in humans, including T126M [235,238]; H201Y and G211R [237]; and A147T and N68S [237] (Figure 5.2).