V (D)J recombination (variable–diversity–joining rearrangement) is the mechanism of somatic recombination that occurs only in developing lymphocytes during the early stages of T and B cell maturation. It results in the highly diverse repertoire of antibodies/immunoglobulins and T cell receptors (TCRs) found in B cells and T cells, respectively.

Ig类别转换 或称 同种型转换 是指一个B细胞克隆在分化过程中重链可变区基因片段（VDJ）保持不变，而按一定顺序与不同的CH基因片段分别发生重排。比较与不同CH基因片段重排后基因编码的重链，它们的可变区相同，而恒定区不同，即识别抗原特异性不变，而Ig ...

这种机制也被称为 12/23规则 (12/23 rule)。 V(D)J重组酶首先在单个12或23 RSSs上组装，产生一个信号复合体(SC)，诱导基因缺口的产生。 与对应RSS的联会(synapsis)可导致配对复合体(PC)的形成， 进而促使切割反应发生（ 图1.2 ）。

2002年4月19日 · V (D)J recombination is of fundamental importance to the generation of diverse antigen receptor repertoires. We review our current understanding of the V (D)J recombination reaction and how it is regulated during lymphocyte development. We also discuss how defects in the mechanism or regulation of V (D)J recombination can lead to human disease.

2024年5月4日 · 资源摘要信息:"2021年3月举行的关于单细胞RNA-Seq数据分析中VDJ分析的高级主题研讨会，该研讨会专注于10x平台产生的单细胞RNA-Seq数据的VDJ分析。 该课程面向具有 单细胞 RNA-Seq 分析 背景的参与者，不涵盖基础知识部分...

In this tutorial, we will work with the Human B cells dataset from a Healthy Donor (1k cells). Watch a short video tutorial or follow the text instructions below. Open up a terminal window. You may log in to a remote server or choose to perform the compute on your local machine. Refer to the System Requirements page for details.

1996年4月5日 · V(D)J recombination requires a pair of signal sequences with spacer lengths of 12 and 23 base pairs. Cleavage by the RAG1 and RAG2 proteins was previously shown to demand only a single signal sequence. Here, we establish conditions where 12- and 23-spacer signal sequences are both necessary for cleavage.

2005年5月15日 · The 12/23 rule prevents rearrangement of V or J genes within their own clusters and ensures the obligatory inclusion of a D segment during IgH gene recombination, because the V H and J H genes are both flanked by 23RS, and the D H genes are flanked by 12RS.

2000年6月1日 · The 12/23 rule, which is mediated at the level of RAG-1/2 recognition and cutting 4,5, specifies that V(D)J recombination occurs only between a gene segment flanked by a 12-RSS and one flanked...

2016年9月29日 · It introduces the basic work flow of how to get information from your next...