
Quantification and characterization of virus-like particles by size ...
2017年3月3日 · Traditionally, transmission electron microscopy (TEM) is used to visualize VLPs and measure particle size. However, with TEM only dried specimens can be visualized. The sample preparation often introduces artefacts or particle deformation which can lead to misinterpretation of particle concentration and particle size [3] , [7] , [8] , [9] .
Virus-like particles: preparation, immunogenicity and their roles as ...
2021年2月25日 · Virus-like particles (VLPs) are virus-derived structures made up of one or more different molecules with the ability to self-assemble, mimicking the form and size of a virus particle but lacking the genetic material so they are not capable of infecting the host cell.
Quality assessment of virus-like particle: A new transmission …
Transmission electron microscopy (TEM) is a gold standard analytical method for nanoparticle characterization and is playing a valuable role in virus-like particle (VLP) characterization extending to other biological entities such as viral vectors.
Virus-like Particles for TEM Regulation and Antitumor Therapy
Recombinant VLP is preferentially taken up by TAM and promotes M1 polarization, thereby enhancing the therapeutic effect on colon cancer and melanoma in mice. Given their small size and ability to cross physiological barriers, VLPs enable …
High-throughput characterization of virus-like particles by …
2016年3月4日 · In this work, we present a size-exclusion ultra-high performance liquid chromatography (SE-UHPLC) method to characterize VLPs and quantify aggregates within 3.1 min per sample applying interlaced injections. Four analytical SEC columns were evaluated for the analysis of human B19 parvo-VLPs and murine polyoma-VLPs.
Immunological and structural evaluation of the intranasally ...
13 小时之前 · Determination of VLP and inactivated virus total protein concentration, dynamic light scattering (DLS) analysis and transmission electron microscopy (TEM) imaging were performed as described 15.
Transmission electron microscopy (TEM) images of viral particles …
We found that MPL/DDA-VLPFMDV could induce strong cell-mediated immune responses by inducing not only VLP-specific IFN-γ+CD4+ (Th1), IL-17A+CD4+ (Th17), and IFN-γ+CD8+ (activated CD8 response) T...
TEM images of our virus-like particles - The Native Antigen Company
2019年9月18日 · Virus-like particles (VLP) are molecules that closely resemble native viruses, but contain no genetic material and are therefore replication-defective and non-infectious. VLPs occur naturally, or can be produced by the expression of individual structural proteins that self-assemble into a virus-like structure.
A systematic approach for scalable purification of virus-like particles
Overview of various virus-like particles (VLPs) expression systems. Downstream process of enveloped and non-enveloped VLPs. Efficient and scalable VLP purification methods. Bioprocess considerations for purifying VLPs. Quality control of VLPs.
Virus-like Particles for TEM Regulation and Antitumor Therapy
2022年12月17日 · Virus-like particles (VLPs) are nanocage structures self-assembled from nucleic acid free viral proteins. Most VLPs range from 20-200 nm in diameter and can naturally drain into lymph nodes to induce robust humoral immunity.