2019年11月12日 · We demonstrated that VPg binds the cap-binding site of eIF4E and forms trimeric complexes with eIF4E and eIF4G; furthermore, we demonstrated that VPg–RNA …

2014年8月1日 · Mutational analyses indicated that the C-terminal region of VPg is important for the VPg-eIF4G interaction; viruses with mutations that alter or disrupt this interaction are …

2014年8月1日 · Mutational analyses indicated that the C-terminal region of VPg is important for the VPg-eIF4G interaction; viruses with mutations that alter or disrupt this interaction are …

2016年1月6日 · Fine mutagenic mapping suggests that the MNV VPg C terminus may interact with eIF4G in a helical conformation. NMR spectroscopy was used to define the VPg binding …

The functional significance of the VPg-eIF4G interaction was shown by the ability of fusion proteins containing the C-terminal peptide of MNV VPg to inhibit in vitro translation of …

2019年11月26日 · The potyvirus-derived viral genome-linked protein (VPg) is covalently bound to the 5' end of viral genomic RNA (gRNA) and associates with host eIF4E for successful …

To dissect these possibilities, we resolved the structure of VPg, revealing a previously unknown 3-dimensional (3D) fold, and characterized the VPg–eIF4E complex using NMR and biophysical …

This approach revealed that VPg binds directly to the eIF4F complex, with a high affinity interaction occurring between VPg and eIF4G. Mutational analyses indicated that the C …

2008年1月18日 · Potyvirus genome linked protein, VPg, interacts with translation initiation factors eIF4E and eIFiso4E, but its role in protein synthesis has not been elucidated. We show that …

We demonstrated that VPg binds the cap-binding site of eIF4E and forms trimeric complexes with eIF4E and eIF4G; furthermore, we demonstrated that VPg–RNA conjugates directly bind …