
稳定表达SLAM受体的Vero和BHK21细胞系的建立及其对犬瘟热病毒分离效果的比较
2018年6月13日 · 研究表明,与SLAM-BHK21细胞相比,SLAM-Vero细胞对CDV的分离率较高,并能产生明显的CPE。 在分离CDV野毒株时,选择肺和脾更容易在SLAM-Vero细胞系上分离出 …
Cytopathic Effect Assay and Plaque Assay to Evaluate in vitro …
Here, we provide the detailed protocol for the cytopathic effect (CPE) assay and the plaque assay for human coronaviruses 229E (HCoV-229E), HCoV-OC43, and HCoV-NL63, to identify novel …
SARS-CoV-2 cytopathic effect (CPE) - SARS-CoV-2 Assays - NCBI Bookshelf
A live SARS-CoV-2 virus assay measuring the ability of compounds to reverse the viral induced cytopathic effect (CPE) in Vero E6 host cells. The CPE reduction assay is a popular and …
Replication kinetics and infectivity of SARS-CoV-2 variants of …
2022年4月26日 · In our experiments with Vero E6 infection, the time point of reaching the highest infectivity correlated well with the visual appearance of CPE for all SARS-CoV-2 variants. This …
ORF3蛋白促进猪流行性腹泻病毒在Vero细胞上的增殖
免疫组化结果表明PEDV ORF3蛋白在Vero细胞中表达;一步生长曲线结果显示虽然带有不同orf3基因的重组病毒感染相同时间的TCID 50 及出现CPE的量不尽相同,在整个培养过程中, …
A live SARS-CoV-2 virus assay measuring the ability of compounds to reverse the viral induced cytopathic e˜ect (CPE) in Vero E6 host cells. The CPE reduction assay is a popular and widely …
现明显细胞病变(cpe)的细胞大多已经出现核质凝缩或形成凋亡小体进入细胞凋亡的过程。 可以断定SARS.CoV 感染VeroE6细胞诱发了细胞凋亡。
登革病毒感染C6/36、Vero、BHK-21和THP-1细胞CPE的比较及病 …
目的比较感染登革病毒(dengue virus,DV)的C6/36,Vero,BHK-21和THP-1细胞的CPE情况,并进行病毒检测.方法将分离自云南西双版纳的Ⅲ型DV中国株D13053和广东省疾病预防控制中心惠赠 …
登革病毒感染C6/36、Vero、BHK-21和THP-1细胞CPE的比较及病 …
目的比较感染登革病毒 (dengue virus,DV)的C6/36、Vero、BHK-21和THP-1细胞的CPE情况,并进行病毒检测。 方法将分离自云南西双版纳的Ⅲ型DV中国株D13053和广东省疾病预防控制中心 …
Development of peptone-based serum-free media to support Vero …
2024年12月30日 · Peptone-based serum-free media provide a robust alternative for supporting Vero CCL-81 cell growth and are suitable for virus production. 1. Introduction. Vero cells, a …
Vero CCL-81 and Calu-3 Cell Lines as Alternative Hosts for …
2023年6月7日 · The replication profile of these isolates was evaluated based on the formation of cytopathic effects (CPE), viral load (Ct value and plaque-forming unit (pfu)), as well as …
Cytopathic effect (CPE) of CE virus on Vero cells. (a ... - ResearchGate
Cytopathic effect (CPE) of CE virus on Vero cells. (a) Negative control noninoculated Vero cells. (b) Infected vero cells showing the progression of CPE at 72 hours post-infection:...
A.5.3 Cytopathic effect (CPE) on Vero cells. (A ... - ResearchGate
Download scientific diagram | A.5.3 Cytopathic effect (CPE) on Vero cells. (A) Uninfected monolayer of Vero cells. (B) VACV replication on Vero cells monolayer showing CPE 48 hr after...
SARS-CoV-2 cytopathic effect (CPE) - PubMed
A live SARS-CoV-2 virus assay measuring the ability of compounds to reverse the viral induced cytopathic effect (CPE) in Vero E6 host cells. The CPE reduction assay is a popular and …
Vero - CCL-81 - ATCC
The Vero cell line was initiated in 1962 from the kidney tissue derived from a normal, adult African green monkey. The cell line can be used in a variety of applications, including the detection of …
In cell culture-based vaccine production, scale-up of adherent cells is challenging. This study shows a process for scaling up adherent Vero cells from static cell factories to influenza …
Improved Zika virus plaque assay using Vero/TMPRSS2 cell line
2024年11月29日 · In Vero/TMPRSS2 cells, the cytopathic effect (CPE) began at 3 dpi, with significant 50–70% CPE occurring at 4 dpi. A significant loss of the cell monolayer, over 90%, …
Ⅲ型登革病毒在C6/36及Vero细胞中复制对其毒力的影响
2019年11月20日 · 目的 探讨Ⅲ型登革病毒(dengue virus,DENV)在C6/36和Vero细胞中复制对其毒力的影响.方法 将DV3-1及DV3-2两株Ⅲ型DENV在C6/36细胞上接种传代培养,待其毒力稳定再 …
Isolation and propagation of Dengue virus in Vero and BHK-21 …
The "standard" methods of isolating dengue virus (DENV) utilize the mosquito cell line C6/36, monkey kidney LLC-MK2 cells, Vero cells, or baby hamster kidney (BHK-21) cells. However, …
Cytopathic Effect (CPE )-Based Drug Screening Assay for SARS …
2022年5月14日 · The cytopathic effect (CPE )-based drug screening is an assay routinely used in the identification of valuable hit compounds for DENV, ZIKV, and SARS-CoV-2 [10,11,12]. In …