2023年5月24日 · 尺寸在纳米范围内的重组抗原特异性单结构域VHH也称为纳米抗体（Nb）或单结构域抗体（sdAb）。 在这篇综述中，我们总结了HCAbs和VHH结构域的主要结构特征，并提供了骆驼科中HCAbs可能产生机制的更新。

A single-domain antibody (sdAb), also known as a Nanobody, is an antibody fragment consisting of a single monomeric variable antibody domain. Like a whole antibody, it is able to bind selectively to a specific antigen.

The camelid-derived single chain antibody (sdAb), also termed VHH or nanobody, is a unique, functional heavy (H)-chain antibody (HCAb). In contrast to conventional antibodies, sdAb is a unique antibody fragment consisting of a heavy-chain variable domain. It lacks light chains and a first constant domain (CH1).

单域抗体 (single-domain antibody，SdAb)只包含单一的抗体可变区（VH/VL）。 同常规的抗体一样，它也能够选择性地结合特定抗原。 单域抗体的分子量仅为12-15 kDa，远小于由两条重链和两条轻链组成的普通抗体（150-160 kDa）。 第一个单域抗体是从骆驼科动物体内发现的。 骆驼的血清中存在一种天然缺失轻链和重链恒定区1 (constant region 1，CH1)的抗体，称为重链抗体 (heavy chain antibody，HcAb)。 其中，位于重链抗体可变区的抗体片段被称为VHH单域抗体，也被称 …

重链抗体（HCAbs）是一类独特的抗体，不含轻链，仅由两条重链组成。它通过重链抗体可变区（VHH）识别抗原，VHH也称单域抗体（sdAb）或纳米抗体（Nb）。在骆驼科动物的血清中，重链抗体甚至能占到总IgG含量的81%，…

2023年6月30日 · Mutating a residue away from the antigen binding site to lysine increased the binding activity of sdAbs. Single-domain antibodies, or VHH, nanobodies, are attractive tools in biotechnology and pharmaceuticals due to their favorable biophysical properties.

2024年8月21日 · Heavy-chain antibodies (HCAbs) are a unique type of antibodies devoid of light chains, and comprised of two heavy chains-only that recognize their cognate antigen by virtue of a single variable domain also referred to as VHH, single domain antibody (sdAb), or nanobody (Nb).

2018年2月18日 · Single-domain antibodies (sdAbs), in particular those engineered from the variable heavy-chain fragment (VHH gene) found in Camelidae heavy-chain antibodies (or IgG2 and IgG3), are the smallest fragments that retain the full antigen-binding capacity of the antibody with advantageous properties as drugs.

纳米抗体(nanobody,Nb)， 即重链单域抗体VHH(variable domain of heavy chain of heavy-chainantibody)——骆驼体内存在着天然缺失轻链的重链抗体(heavy-chain antibody,HCAb)，克隆其可变区VHH而得到的只由一个重链可变区组成的单域抗体，是目前可以得到的具有完整功能的稳 …

2024年7月31日 · Single-domain antibodies (sdAbs) are the smallest functional antibody format (~ 15 kDa) and represent a viable alternative to IgG in many applications. However, unlike...